Prevalence of Antibodies to Trypanosoma cruzi, Toxoplasma gondii, Encephalitozoon cuniculi, Sarcocystis neurona, Besnoitia darlingi, and Neospora caninum in North American Opossums, Didelphis virginiana, from Southern Louisiana
Dubey, J. P.
Houk, Alice E.
Goodwin, David G.
Zajac, Anne M.
Barr, Stephen C.
Lindsay, David S.
MetadataShow full item record
We examined the prevalence of antibodies to zoonotic protozoan parasites (Trypanosoma cruzi, Toxoplasma gondii, and Encephalitozoon cuniculi) and protozoans of veterinary importance (Neospora caninum, Sarcocystis neurona, and Besnoitia darlingi) in a population of North American opossums (Didelphis virginiana) from Louisiana. Samples from 30 opossums were collected as part of a survey for T. cruzi in Louisiana. Frozen sera from these 30 opossums were examined using an indirect immunofluorescent antibody test (IFAT) against in vitro-produced antigenic stages of these protozoans. Additionally, 24 of the 30 samples were examined using hemoculture, and all 30 were examined in the modified direct agglutination test (MAT) for antibodies to To. gondii. The prevalences of reactive IFAT samples were as follows: 60% for T. cruzi, 27% for To. gondii, 23% for E. cuniculi, 17% for S. neurona, 47% for B. darlingi, and 0% for N. caninum. Hemoculture revealed that 16 (67%) of 24 samples were positive for T. cruzi, compared to 18 of 30 (60%) by IFAT. The sensitivity and specificity for the IFAT compared to hemoculture was 100% for each. The modified direct agglutination test revealed that 9 (30%) of the 30 samples from opossums had antibodies to To. gondii, compared to 8 (27%) using the IFAT. The sensitivity and specificity of the IFAT compared to the MAT was 100% and 72%, respectively.
Showing items related by title, author, creator and subject.
Functional genomics of a generalist parasitic plant: Laser microdissection of host-parasite interface reveals host-specific patterns of parasite gene expression Honaas, Loren A; Wafula, Eric K; Yang, Zhenzhen; Der, Joshua P; Wickett, Norman J; Altman, Naomi S; Taylor, Christopher G; Yoder, John I; Timko, Michael P; Westwood, James H; dePamphilis, Claude W (2013-01-09)Background Orobanchaceae is the only plant family with members representing the full range of parasitic lifestyles plus a free-living lineage sister to all parasitic lineages, Lindenbergia. A generalist member of this ...
Lucas, A. S.; Swecker, W. S.; Scaglia, G.; Lindsay, D. S.; Zajac, A. M. (American Society of Parasitology, 2006-10)Rectal fecal samples were collected daily on 10 consecutive days in November 2004 from I I weaned beef heifers to assess daily variation in fecal oocyst count and species composition. Subsequent samples were collected from ...
Development of Sarcocystis falcatula in cell cultures demonstrates that it is different from Sarcocystis neurona Lindsay, D. S.; Dubey, J. P.; Horton, K. M.; Bowman, D. D. (Cambridge University Press, 1999-03)The development of Sarcocystis falcatula merozoites in bovine turbinate (BT) cell cultures is described and compared with development of Sarcocystis neurona merozoites. Merozoites of S. falcatula entered BT cell cultures ...