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dc.contributorVirginia Techen
dc.contributor.authorDubey, J. P.en
dc.contributor.authorHouk, Alice E.en
dc.contributor.authorGoodwin, David G.en
dc.contributor.authorZajac, Anne M.en
dc.contributor.authorBarr, Stephen C.en
dc.contributor.authorLindsay, David S.en
dc.date.accessioned2014-06-20T14:13:21Zen
dc.date.available2014-06-20T14:13:21Zen
dc.date.issued2010-12en
dc.identifier.citationAlice E. Houk, David G. Goodwin, Anne M. Zajac, Stephen C. Barr, J. P. Dubey, and David S. Lindsay (2010). "Prevalence of Antibodies to Trypanosoma cruzi, Toxoplasma gondii, Encephalitozoon cuniculi, Sarcocystis neurona, Besnoitia darlingi, and Neospora caninum in North American Opossums, Didelphis virginiana, from Southern Louisiana," Journal of Parasitology, Vol. 96, Issue 6, pp. 1119-1122. doi: http://dx.doi.org/10.1645/GE-2515.1en
dc.identifier.issn0022-3395en
dc.identifier.urihttp://hdl.handle.net/10919/49077en
dc.description.abstractWe examined the prevalence of antibodies to zoonotic protozoan parasites (Trypanosoma cruzi, Toxoplasma gondii, and Encephalitozoon cuniculi) and protozoans of veterinary importance (Neospora caninum, Sarcocystis neurona, and Besnoitia darlingi) in a population of North American opossums (Didelphis virginiana) from Louisiana. Samples from 30 opossums were collected as part of a survey for T. cruzi in Louisiana. Frozen sera from these 30 opossums were examined using an indirect immunofluorescent antibody test (IFAT) against in vitro-produced antigenic stages of these protozoans. Additionally, 24 of the 30 samples were examined using hemoculture, and all 30 were examined in the modified direct agglutination test (MAT) for antibodies to To. gondii. The prevalences of reactive IFAT samples were as follows: 60% for T. cruzi, 27% for To. gondii, 23% for E. cuniculi, 17% for S. neurona, 47% for B. darlingi, and 0% for N. caninum. Hemoculture revealed that 16 (67%) of 24 samples were positive for T. cruzi, compared to 18 of 30 (60%) by IFAT. The sensitivity and specificity for the IFAT compared to hemoculture was 100% for each. The modified direct agglutination test revealed that 9 (30%) of the 30 samples from opossums had antibodies to To. gondii, compared to 8 (27%) using the IFAT. The sensitivity and specificity of the IFAT compared to the MAT was 100% and 72%, respectively.en
dc.description.sponsorshipOffice of Research and Graduate Studies Virginia-Maryland Regional College of Veterinary Medicine grant 117797en
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.publisherAmerican Society of Parasitologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectparasites diseases and disordersen
dc.subjectparasitesen
dc.subjecthostsen
dc.subjectland zonesen
dc.subjectnearcticen
dc.subjectregionen
dc.subjectnorth americaen
dc.subjectusaen
dc.subjectbesnoitia darlingien
dc.subjectencephalitozoon cuniculien
dc.subjectneospora caninumen
dc.subjectsarcocystis neuronaen
dc.subjecttoxoplasma gondiien
dc.subjecttrypanosoma cruzi [mammalianen
dc.subjecthosts / didelphis virginiana / prevalence] [louisiana / south / ].en
dc.subjectdidelphis virginiana [protozoan parasites / besnoitia darlingi /en
dc.subjectprevalence] [ / encephalitozoon cuniculi / ] [ / neospora caninum / ] [en
dc.subject/ sarcocystis neurona / ] [ / toxoplasma gondii / ] [ / trypanosomaen
dc.subjectcruzi / ] [louisiana / south / ].en
dc.subjectchordatesen
dc.subjectmammalsen
dc.subjectmarsupialsen
dc.subjectprotozoansen
dc.subjectvertebratesen
dc.subjectanimalia, chordata, vertebrata, mammalia, marsupialia, didelphidae,en
dc.subjectprotozoa, apicomplexa, sporozoea, coccidia, microspora,en
dc.subjectsarcomastigophora, mastigophora, zoomastigophorea, kinetoplastidaen
dc.subjectdidelphis virginiana (didelphidae): [host].en
dc.subjectbesnoitia darlingi (coccidia): [parasite].en
dc.subjectneospora caninum (coccidia): [parasite].en
dc.subjectsarcocystis neurona (coccidia): [parasite].en
dc.subjecttoxoplasma gondii (coccidia): [parasite].en
dc.subjectencephalitozoon cuniculi (microspora): [parasite].en
dc.subjecttrypanosoma cruzi (kinetoplastida): [parasite].en
dc.titlePrevalence of Antibodies to Trypanosoma cruzi, Toxoplasma gondii, Encephalitozoon cuniculi, Sarcocystis neurona, Besnoitia darlingi, and Neospora caninum in North American Opossums, Didelphis virginiana, from Southern Louisianaen
dc.typeArticle - Refereeden
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-2515.1en
dc.date.accessed2014-06-16en
dc.title.serialJournal of Parasitologyen
dc.identifier.doihttps://doi.org/10.1645/ge-2515.1en
dc.type.dcmitypeTexten


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