Expression of a heterologous protein C in mammary tissue of transgenic animals using a long whey acidic protein promoter

Publication Date
2001-07-17Filing Date
1999-05-28Inventor
Drohan, William N.
Velander, William H.
Lubon, Henryk
Hennighausen, Lothar
Metadata
Show full item recordAbstract
An isolated DNA sequence which regulates the expression of a heterologous gene composed of a mouse whey acidic protein promoter having a length of greater than about 2.4 kb extending upstream from the unique KpnI site in the mouse whey acidic protein gene is disclosed. Specifically a mouse whey acidic protein promoter of about 4.1-4.2 kb in length extending upstream from the unique KpnI site is preferred. This mouse whey acid protein promoter is operably linked to a DNA sequence encoding a heterologous polypeptide and used to prepare transgenic non-human mammals expressing the heterologous polypeptide in their milk. Particularly efficient expression of both cDNAs and genomic DNAs encoding heterologous polypeptides was obtained in transgenic non-human mammals using this promoter, known as the long whey acidic protein promoter.
Assignee
The American Red Cross
Virginia Tech Intellectual Properties, Inc.
The United States of America as represented by the Department of Health and Human Services
Patent Number
6262336
Application Number
9321831
Primary/U.S. Class
536/24.1
Other/U.S. Class
800/14
800/7
800/15
800/16
800/17
800/18
CPC Class
A01K67/0275
A01K2207/15
A01K2217/00
A01K2217/05
A01K2227/10
A01K2227/105
A01K2227/108
A01K2267/01
A61K38/00
C07K14/75
C07K2319/00
C07K2319/02
C12N15/8509
C12N9/6464
C12Y304/21069
C12N2830/008
Patent Type
utility
Collections
- Virginia Tech Patents [687]