We have demonstrated the fabrication of long period gratings in random hole optical fibers. The long period gratings are fabricated by a point-by-point technique using a CO2 laser. The gratings with a periodicity of 450 µm are fabricated and a maximum coupling efficiency of −9.81 dB has been achieved. Sensing of different refractive indices in the surrounding mediums is demonstrated by applying standard liquids with refractive indices from 1.400 to 1.440 to the long period grating.