VTechWorks staff will be away for the Thanksgiving holiday starting Wednesday afternoon, Nov. 25, through Sunday Nov. 29, and will not be replying to requests during this time. Thank you for your patience.

Show simple item record

dc.contributor.authorThayagan, Sathieseelan
dc.date.accessioned2018-04-13T20:41:06Z
dc.date.available2018-04-13T20:41:06Z
dc.date.issued2018-03-12
dc.identifier.urihttp://hdl.handle.net/10919/82831
dc.description.abstractE.coli 0157:H7 is one of the major bacterial diseases transmitted through drinking water and is a major contaminant on leafy greens. Results from current traditional plating methods including P-A analysis is time consuming and this slow process is an hinderance as a preventive control for food-borne outbreaks. In this experiment a fluorescence immonoassay outlined in fig. 7, is proposed for detecting E.coli 0157:H7 in water with a moderate amount of preparatory steps and less than 5 minutes for the detection of this pathogen. In examining the relationship between E.coli 0157: H7 CFU/0.1mL to fluorescence intensity, our model data evaluated with Minitab 16, revealed a P(0.001)= 0, and F=271.56 demonstrating a stong relationship of E.coli 0157:H7 detection with this immunoassay. E.aerogenes the negative contol for the immunoassay revealed a P(0.001)=0.677 and F=0.31, validating the specificity of the model. E.coli 0157:H7 was detected at 1 CFU/0.1mL with the proposed model and least three sigma (3σ) levels above the baseline for the control blank, thereby showing ≥ 99.7% probability of locating E.coli 0157:H7 when compared to the experiment control.en_US
dc.language.isoenen_US
dc.publisherVirginia Techen_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 United Statesen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/en
dc.titleRapid detection of Escherichia coli in field based potable water applicationsen_US
dc.typeReporten_US
dc.contributor.departmentFood Science and Technologyen_US
dc.description.degreeMALSen_US
thesis.degree.nameMaster of Agricultural and Life Sciencesen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineFood Safety and Biosecurityen_US
dc.contributor.committeechairStrawn, Laura K.
dc.contributor.committeememberStrawn, Laura K.
dc.contributor.committeememberEifert, Joseph D.
dc.contributor.committeememberWilliams, Robert
dc.subject.cabtQdot, streptavidin, E.coli 0157:H7, Escherichia coli, fluorescenceen_US


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Creative Commons Attribution-NonCommercial-NoDerivs 3.0 United States
License: Creative Commons Attribution-NonCommercial-NoDerivs 3.0 United States