Polyvinylidene Fluoride (PVDF) piezoelectric electrospun nanofibers have been intensively used for sensing and actuation applications in the last decade. However, in most cases, random PVDF piezoelectric nanofiber mats have moderate piezoelectric response compared to aligned PVDF nanofibers. In this work, we demonstrate the effect of alignment conducted by a collector setup composed of two-metal bars with gab inside where the aligned fiber can be formed. That is what we called static aligned nanofibers, which is distinct from the dynamic traditional technique using a high speed rotating drum. The two-bar system shows a superior alignment degree for the PVDF nanofibers. Also, the effect of added carbon nanotubes (CNTs) of different concentrations to PVDF nanofibers is studied to observe the enhancement of piezoelectric response of PVDF nanofibers. Improvement of β-phase content of aligned (PVDF) nanofibers, as compared to randomly orientated fibers, is achieved. Significant change in the piezoelectricity of PVDF fiber is produced with added CNTs with saturation response in the case of 0.3 wt % doping of CNTs, and piezoelectric sensitivity of 73.8 mV/g with applied masses down to 100 g.