Show simple item record

dc.contributor.authorShao, Fangen
dc.contributor.authorWang, Xingguoen
dc.contributor.authorYu, Jianfengen
dc.contributor.authorJiang, Honglinen
dc.contributor.authorZhu, Binen
dc.contributor.authorGu, Zhiliangen
dc.date.accessioned2018-10-10T19:22:27Zen
dc.date.available2018-10-10T19:22:27Zen
dc.date.issued2014-03-13en
dc.identifier.othere91236en
dc.identifier.urihttp://hdl.handle.net/10919/85339en
dc.description.abstractThe sterol regulatory element binding transcription factor 2 (SREBF2) gene encodes a transcription factor that activates the expression of many genes involved in the synthesis and uptake of cholesterol, fatty acids, triglycerides, and phospholipids. Through bioinformatics, we found that intron 16 of the chicken SREBF2 gene might encode the chicken miR-33. Using quantitative RT-PCR, we detected the expression of miR-33 in a variety of chicken tissues including skeletal muscle, adipose tissue, and liver. Three hundred and seventy eight genes were predicted to be potential targets of miR-33 in chickens via miRNA target prediction programs “miRanda” and “TargetScan”. Among these targets, the gene FTO (fat mass and obesity associated) encodes a Fe(II)- and 2-oxoglutarate-dependent nucleic acid demethylase that regulates lipid metabolism, and the possibility that its expression is negatively regulated by miR-33 in the chicken liver was therefore further studied. Co-transfection and dual-luciferase reporter assays showed that the expression of luciferase reporter gene linked to the 3′-untranslated region (3′UTR) of the chicken FTO mRNA was down-regulated by overexpression of the chicken miR-33 in the C2C12 cells (P<0.05). Furthermore, this down-regulation was completely abolished when the predicted miR-33 target site in the FTO 3′UTR was mutated. In contrast, the expression of FTO mRNA in the primary chicken hepatocytes was up-regulated after transfection with the miR-33 inhibitor LNA-anti-miR-33. Using quantitative RT-PCR, we also found that the expression of miR-33 was increased in the chicken liver from day 0 to day 49 of age, whereas that of the FTO mRNA was decreased during the same age period. These data together suggest that miR-33 might play an important role in lipid metabolism in the chicken liver by negatively regulating the expression of the FTO gene.en
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleExpression of miR-33 from an SREBF2 Intron Targets the FTO Gene in the Chickenen
dc.typeArticle - Refereeden
dc.description.versionPeer Revieweden
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.title.serialPLOS ONEen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0091236en
dc.identifier.volume9en
dc.identifier.issue3en
dc.type.dcmitypeTexten
dc.identifier.pmid24626192en
dc.identifier.eissn1932-6203en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International