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dc.contributor.authorGilmore, Brian L.
dc.contributor.authorWinton, Carly E.
dc.contributor.authorDemmert, Andrew C.
dc.contributor.authorTanner, Justin R.
dc.contributor.authorBowman, Sam
dc.contributor.authorKarageorge, Vasilea
dc.contributor.authorPatel, Kaya
dc.contributor.authorSheng, Zhi
dc.contributor.authorKelly, Deborah F.
dc.date.accessioned2019-01-24T18:24:47Z
dc.date.available2019-01-24T18:24:47Z
dc.date.issued2015-09-23
dc.identifier.issn2045-2322
dc.identifier.other14440
dc.identifier.urihttp://hdl.handle.net/10919/86881
dc.description.abstractWe present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.en_US
dc.description.sponsorshipVirginia Tech; Institute for Critical Technology and Applied Science (ICTAS) at Virginia Tech; Commonwealth Health Research Board [2080914]; Concern Foundation [303872]; NCI/NIH [R01CA193578]; ICTAS Doctoral Scholar's program at Virginia Tech; Medical Research Scholar's program at the Virginia Tech Carilion Research Institute
dc.format.extent8
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherSpringer Nature
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectrna-polymerase-ii
dc.subjectelectron-microscopy
dc.subjectbreast-cancer
dc.subjectterminal domain
dc.subjectaffinity grids
dc.subjectbrca1
dc.subjecttranscription
dc.subjectholoenzyme
dc.subjectresistance
dc.titleA Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Diseaseen_US
dc.typeArticle - Refereed
dc.description.notesThis research is supported by funds from Virginia Tech, the Institute for Critical Technology and Applied Science (ICTAS) at Virginia Tech, the Commonwealth Health Research Board [2080914], the Concern Foundation [303872], and NCI/NIH [R01CA193578] to D.F.K. C.W. is funded through the ICTAS Doctoral Scholar's program at Virginia Tech and the Medical Research Scholar's program at the Virginia Tech Carilion Research Institute.
dc.title.serialScientific Reports
dc.identifier.doihttps://doi.org/10.1038/srep14440
dc.identifier.volume5
dc.type.dcmitypeText
dc.identifier.pmid26395823


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International