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The response of rat thymus nuclei to thyroid hormones

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dc.contributor.authorRuark, Edwin Warrenen
dc.contributor.departmentBiochemistry and Nutritionen
dc.date.accessioned2014-03-14T21:08:49Zen
dc.date.adate2010-01-13en
dc.date.available2014-03-14T21:08:49Zen
dc.date.issued1970en
dc.date.rdate2010-01-13en
dc.date.sdate2010-01-13en
dc.description.abstractThe effect of triiodothyronine (L-T₃) on the protein, RNA and DNA fractions of the thymus nuclei of growth-arrested sulfaguanidine-fed rats has been studied. A single dose of 15 µg of L-T₃ stimulated the incorporation of ¹⁴C-labelled amino acids into total nuclear protein, whole histone and histone fractions. The incorporation of the labelled amino acid into nuclear protein was increased 40 percent over the control 4 hours after the administration of L-T₃. The incorporation of alanine-1-¹⁴C into the total histone fraction reached a maximum 4 hours after the administration of L-T₃ and then decreased 8 and 16 hours after the administration of L-T₃. The incorporation of alanine-1-¹⁴C into histone fraction f₁ reached a maximum at 4 hours after the administration of L-T₃ while the incorporation into fractions f<sub>2a</sub>, f<sub>2b</sub> and f₃ did not reach a maximum until 8 hours after the administration of L-T₃. Time course studies showed that the protein and RNA moieties of thymus chromatin of rats injected with a single dose of L-T₃ was increased to a maximum at 4 hours. The T<sub>m</sub> of chromatin was increased 2° by L-T₃ administration as early as 2 hours. However, the T<sub>m</sub> of DNA was not affected. Four hours after the administration of L-T₃ the template efficiency increased 171 percent. The template efficiency of DNA was not affected by L-T₃. These results demonstrate that thyroid hormones modify the chromatin in such a way that the template efficiency is increased. An hypothesis for the mechanism of thyroid hormone action was presented.en
dc.description.degreePh. D.en
dc.format.extent89 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-01132010-020032en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-01132010-020032/en
dc.identifier.urihttp://hdl.handle.net/10919/37198en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1970.R8.pdfen
dc.relation.isformatofOCLC# 22366655en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1970.R8en
dc.subject.lcshRatsen
dc.subject.lcshThymusen
dc.subject.lcshThyroid hormonesen
dc.titleThe response of rat thymus nuclei to thyroid hormonesen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Nutritionen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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