FusoPortal: an Interactive Repository of Hybrid MinIONSequenced Fusobacterium Genomes Improves Gene Identification and Characterization

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dc.contributor.authorSanders, Blake E.en
dc.contributor.authorUmaƱa, Arianaen
dc.contributor.authorLemkul, Justin A.en
dc.contributor.authorSlade, Daniel J.en
dc.contributor.departmentBiochemistryen
dc.date.accessioned2018-07-24T17:01:39Zen
dc.date.available2018-07-24T17:01:39Zen
dc.date.issued2018-07-05en
dc.description.abstractHere we present FusoPortal, an interactive repository of Fusobacterium genomes that were sequenced using a hybrid MinION long-read sequencing pipeline, followed by assembly and annotation using a diverse portfolio of predominantly open-source software. Significant efforts were made to provide genomic and bioinformatic data as downloadable files, including raw sequencing reads, genome maps, gene annotations, protein functional analysis and classifications, and a custom BLAST server for FusoPortal genomes. FusoPortal has been initiated with eight complete genomes, of which seven were previously only drafts that ranged from 24 to 67 contigs. We have showcased that the genomes in FusoPortal provide accurate open reading frame annotations and have corrected a number of large (3-kb) genes that were previously misannotated due to contig boundaries. In summary, FusoPortal (http://fusoportal.org) is the first database of MinION-sequenced and completely assembled Fusobacterium genomes, and this central Fusobacterium genomic and bioinformatic resource will aid the scientific community in developing a deeper understanding of how this human pathogen contributes to an array of diseases, including periodontitis and colorectal cancer.en
dc.description.sponsorshipUSDA National Institute of Food and Agricultureen
dc.description.sponsorshipVirginia Tech Department of Biochemistryen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1128/mSphere.00228-18en
dc.identifier.issue4en
dc.identifier.urihttp://hdl.handle.net/10919/84327en
dc.identifier.volume3en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectFusobacteriumen
dc.subjectFusobacterium nucleatumen
dc.subjectpathogenesisen
dc.subjectcolon canceren
dc.subjectcolorectal canceren
dc.subjectgenomicsen
dc.subjecthost-pathogenen
dc.titleFusoPortal: an Interactive Repository of Hybrid MinIONSequenced Fusobacterium Genomes Improves Gene Identification and Characterizationen
dc.title.serialmSphereen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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