Validation of DNA marker-assisted selection for forage biomass productivity under deficit irrigation in alfalfa

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dc.contributor.authorSingh, Lovepreeten
dc.contributor.authorPierce, Chrisen
dc.contributor.authorSantantonio, Nicholasen
dc.contributor.authorSteiner, Roberten
dc.contributor.authorMiller, Donen
dc.contributor.authorReich, Jonen
dc.contributor.authorRay, Ianen
dc.date.accessioned2022-07-26T15:59:07Zen
dc.date.available2022-07-26T15:59:07Zen
dc.date.issued2022-03en
dc.description.abstractDrought and limited irrigation resources threaten agricultural sustainability in many regions of the world. Application of genomic-based breeding strategies may benefit crop variety development for these environments. Here, we provide a first report on the effect of deploying DNA marker-assisted selection (MAS) for the drought resilience quantitative trait in alfalfa (Medicago sativa L.). The goals of this study were to validate the effect of several quantitative trait loci (QTL) associated with alfalfa forage and crown-root (CR) biomass during drought and to determine their potential to improve forage yield of elite germplasm under water-limited conditions. Marker assisted selection was employed to introgress favorable or unfavorable DNA marker alleles affiliated with 10 biomass QTL into three elite backgrounds. Thirty-two populations were developed and evaluated for forage productivity over 3 yr under continuous deficit irrigation management in New Mexico, USA. Significant yield differences (ranging from -13 to 26%) were detected among some MAS-derived populations in all three elite backgrounds. Application of QTL MAS generally resulted in expected phenotypic responses within an elite genetic background that was similar to that in which the QTL were originally identified. However, relative performance of the populations varied substantially across the three genetic backgrounds. These outcomes indicate that QTL MAS can significantly affect forage productivity of elite alfalfa germplasm in drought-stressed environments. However, if biomass QTL are detected in donor germplasm that is genetically dissimilar to targeted elite populations, characterization of donor alleles may be warranted within elite backgrounds of interest to confirm their phenotypic effects prior to implementing MAS-based breeding.en
dc.description.notesThis research was supported by USDA Hatch funds provided to the New Mexico Agriculture Experiment Station; a Cal/West Seeds grant to IMR; USDA-NRI grant 2005-35100-16268 to IMR; and USDA grant (No. 2010-34186-20979) to IMR via the Southwest Consortium on Plant Genetics and Water Resources. Special thanks to Gina Babb for technical assistance affiliated with DNA marker genotyping of the full-sib C<INF>0</INF> population and production of hand-crossed seed for the 32 populations evaluated. We also gratefully acknowledge Greg Bettmann, Irene Calderon, Lei E, Nina Klypina, Johnny Maruthavanan, Chris Meenach, Chris Pierce, Mat Pinch, Austin Ray, Karen Ray, Nicole Ray, and Tracy Sterling for their tremendous support toward collecting crown-root biomass data from the CHBC<INF>1</INF> population under extreme summer field conditions of 2007. Valuable comments from two anonymous reviewers are gratefully acknowledged.en
dc.description.sponsorshipUSDA Hatch funds; Cal/West Seeds grant; USDA-NRI grant [2005-35100-16268]; USDA via the Southwest Consortium on Plant Genetics and Water Resources [2010-34186-20979]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/tpg2.20195en
dc.identifier.eissn1940-3372en
dc.identifier.issue1en
dc.identifier.othere20195en
dc.identifier.pmid35178866en
dc.identifier.urihttp://hdl.handle.net/10919/111373en
dc.identifier.volume15en
dc.language.isoenen
dc.publisherWileyen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectwater-use efficiencyen
dc.subjectdrought toleranceen
dc.subjectyielden
dc.subjectregistrationen
dc.subjectimprovementen
dc.subjectgermplasmen
dc.subjectgenomicsen
dc.subjectcarbonen
dc.titleValidation of DNA marker-assisted selection for forage biomass productivity under deficit irrigation in alfalfaen
dc.title.serialPlant Genomeen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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Destination Area: Global Systems Science (GSS)