Strong Purifying Selection Is Associated with Genome Streamlining in Epipelagic Marinimicrobia

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dc.contributor.authorMartinez-Gutierrez, Carolina Alejandraen
dc.contributor.authorAylward, Frank O.en
dc.date.accessioned2021-10-11T12:42:17Zen
dc.date.available2021-10-11T12:42:17Zen
dc.date.issued2019-10-01en
dc.date.updated2021-10-11T12:42:15Zen
dc.description.abstractMarine microorganisms inhabiting nutrient-depleted waters play critical roles in global biogeochemical cycles due to their abundance and broad distribution. Many of these microbes share similar genomic features including small genome size, low % G + C content, short intergenic regions, and low nitrogen content in encoded amino acid residue side chains (N-ARSC), but the evolutionary drivers of these characteristics are unclear. Here, we compared the strength of purifying selection across the Marinimicrobia, a candidate phylum which encompasses a broad range of phylogenetic groups with disparate genomic features, by estimating the ratio of nonsynonymous and synonymous substitutions (dN/dS) in conserved marker genes. Our analysis reveals that epipelagic Marinimicrobia that exhibit features consistent with genome streamlining have significantly lower dN/dS values when compared with their mesopelagic counterparts. We also found a significant positive correlation between median dN/dS values and % G + C content, N-ARSC, and intergenic region length. We did not identify a significant correlation between dN/dS ratios and estimated genome size, suggesting the strength of selection is not a primary factor shaping genome size in this group. Our findings are generally consistent with genome streamlining theory, which postulates that many genomic features of abundant epipelagic bacteria are the result of adaptation to oligotrophic nutrient conditions. Our results are also in agreement with previous findings that genome streamlining is common in epipelagic waters, suggesting that microbes inhabiting this region of the ocean have been shaped by strong selection together with prevalent nutritional constraints characteristic of this environment.en
dc.description.versionPublished versionen
dc.format.extentPages 2887-2894en
dc.format.extent8 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/gbe/evz201en
dc.identifier.eissn1759-6653en
dc.identifier.issn1759-6653en
dc.identifier.issue10en
dc.identifier.orcidAylward, Frank [0000-0002-1279-4050]en
dc.identifier.other5572122 (PII)en
dc.identifier.pmid31539038en
dc.identifier.urihttp://hdl.handle.net/10919/105225en
dc.identifier.volume11en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000504448300016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectEvolutionary Biologyen
dc.subjectGenetics & Heredityen
dc.subjectgenome streamliningen
dc.subjectpurifying selectionen
dc.subjectevolutionary genomicsen
dc.subjectdN/dS ratioen
dc.subjectMarinimicrobiaen
dc.subjectuncultured picoplanktonen
dc.subjectCLOSELY-RELATED BACTERIALen
dc.subjectPOPULATION-SIZEen
dc.subjectEVOLUTIONen
dc.subjectALIGNMENTSen
dc.subjectDIVERGENCEen
dc.subjectDYNAMICSen
dc.subjectPATTERNSen
dc.subjectTOOLen
dc.subjectDevelopmental Biologyen
dc.subject0601 Biochemistry and Cell Biologyen
dc.subject0603 Evolutionary Biologyen
dc.subject0604 Geneticsen
dc.subject.meshBacteriaen
dc.subject.meshGenetic Markersen
dc.subject.meshEvolution, Molecularen
dc.subject.meshGenome, Bacterialen
dc.subject.meshSelection, Geneticen
dc.titleStrong Purifying Selection Is Associated with Genome Streamlining in Epipelagic Marinimicrobiaen
dc.title.serialGenome Biology and Evolutionen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2019-09-08en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen

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