Creation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technology

dc.contributor.authorHan, Jiananen
dc.contributor.authorGuo, Bingfuen
dc.contributor.authorGuo, Yongen
dc.contributor.authorZhang, Boen
dc.contributor.authorWang, Xiaoboen
dc.contributor.authorQiu, Li-Juanen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-02-05T17:47:33Zen
dc.date.available2020-02-05T17:47:33Zen
dc.date.issued2019-11-22en
dc.description.abstractSoybean is an important economic crop and a typical short-day crop, sensitive to photoperiod, and has narrow geographical adaptative region, which limit the creation of transgenic materials and reduce the breeding efficiency of new varieties. In addition, the genetic transformation efficiency of soybean is lower than that of many other crops, and the available receptor genotypes are limited. In this study, Agrobacterium-mediated transformation were used to introduce the CRISPR/Cas9 expression vector into soybean cultivar Jack and generated targeted mutants of E1 gene controlling soybean flowering. We obtained two novel types of mutations, 11 bp and 40 bp deletion at E1 coding region, respectively, and frameshift mutations produced premature translation termination codons and truncated E1 proteins, causing obvious early flowering under long day condition. In addition, no off-target effects were observed by predicting and analyzing the potential off-target sites of E1 targets. Significant decreased E1 gene expression of two novel mutants showed that the truncated E1 protein disinhibited GmFT2a/5a and increasing GmFT2a/5a gene expressions resulted obvious early flowering. Homozygous trans-clean mutants without T-DNA elements were also obtained and showed early flowering under long day condition. The photo-insensitive soybean transformation receptor we created laid a foundation for breeding excellent transgenic receptors suitable for high latitudes.en
dc.description.notesThis work was supported by the National Science and Technological innovation Program of China.en
dc.description.sponsorshipNational Science and Technological innovation Program of Chinaen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fpls.2019.01446en
dc.identifier.issn1664-462Xen
dc.identifier.other1446en
dc.identifier.pmid31824524en
dc.identifier.urihttp://hdl.handle.net/10919/96723en
dc.identifier.volume10en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsoybeanen
dc.subjectCRISPRen
dc.subjectCas9en
dc.subjectgene editingen
dc.subjectE1en
dc.subjectAgrobacterium-mediated transformationen
dc.titleCreation of Early Flowering Germplasm of Soybean by CRISPR/Cas9 Technologyen
dc.title.serialFrontiers in Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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