Aedes aegypti sialokinin facilitates mosquito blood feeding and modulates host immunity and vascular biology

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dc.contributor.authorMartin-Martin, Inesen
dc.contributor.authorLeon, Paola Carolina Valenzuelaen
dc.contributor.authorAmo, Lauraen
dc.contributor.authorShrivastava, Gauraven
dc.contributor.authorIniguez, Evaen
dc.contributor.authorAryan, Azadehen
dc.contributor.authorBrooks, Stevenen
dc.contributor.authorKojin, Bianca B.en
dc.contributor.authorWilliams, Adeline E.en
dc.contributor.authorBolland, Silviaen
dc.contributor.authorAckerman, Hansen
dc.contributor.authorAdelman, Zach N.en
dc.contributor.authorCalvo, Ericen
dc.date.accessioned2022-07-08T16:27:37Zen
dc.date.available2022-07-08T16:27:37Zen
dc.date.issued2022-04-12en
dc.description.abstractSaliva from mosquitoes contains vasodilators that antagonize vasoconstrictors produced at the bite site. Sialokinin is a vasodilator present in the saliva of Aedes aegypti. Here, we investigate its function and describe its mechanism of action during blood feeding. Sialokinin induces nitric oxide release similar to substance P. Sialokinin-KO mosquitoes produce lower blood perfusion than parental mosquitoes at the bite site during probing and have significantly longer probing times, which result in lower blood feeding success. In contrast, there is no difference in feeding between KO and parental mosquitoes when using artificial membrane feeders or mice that are treated with a substance P receptor antagonist, confirming that sialokinin interferes with host hemostasis via NK1R signaling. While sialokinin-KO saliva does not affect virus infection in vitro, it stimulates macrophages and inhibits leukocyte recruitment in vivo. This work highlights the biological functionality of salivary proteins in blood feeding.en
dc.description.notesWe thank Brian Bonilla for excellent technical work with mosquitoes; Andre Laughinghouse, Kevin Lee, and Yonas Gebremicale for mosquito rearing; Van My Pham and Karina Sewell for excellent salivary gland dissections; and Glenn Nardone and Lisa Olano for MS/MS analysis. This research was supported by the Division of Intramural Research Program of the NIH/NIAID (AI001246, to E.C.) and by a subcontract from grant 1R01AI099483 (to Z.N.A.).en
dc.description.sponsorshipDivision of Intramural Research Program of the NIH/NIAID [AI001246, 1R01AI099483]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.celrep.2022.110648en
dc.identifier.issn2211-1247en
dc.identifier.issue2en
dc.identifier.other110648en
dc.identifier.pmid35417706en
dc.identifier.urihttp://hdl.handle.net/10919/111181en
dc.identifier.volume39en
dc.language.isoenen
dc.publisherCell Pressen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectyellow-fever mosquitoen
dc.subjectsubstance-pen
dc.subjectsalivary-glandsen
dc.subjectvasodilatory peptideen
dc.subjectup-regulationen
dc.subjectexpressionen
dc.subjectvectoren
dc.subjectflyen
dc.subjectneuropeptideen
dc.subjectanophelesen
dc.titleAedes aegypti sialokinin facilitates mosquito blood feeding and modulates host immunity and vascular biologyen
dc.title.serialCell Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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