Biogenic formation of amorphous carbon by anaerobic methanotrophs and select methanogens
| dc.contributor.author | Allen, Kylie D. | en |
| dc.contributor.author | Wegener, Gunter | en |
| dc.contributor.author | Sublett, D. Matthew, Jr. | en |
| dc.contributor.author | Bodnar, Robert J. | en |
| dc.contributor.author | Feng, Xu | en |
| dc.contributor.author | Wendt, Jenny | en |
| dc.contributor.author | White, Robert H. | en |
| dc.date.accessioned | 2021-11-10T14:50:36Z | en |
| dc.date.available | 2021-11-10T14:50:36Z | en |
| dc.date.issued | 2021-10-27 | en |
| dc.description.abstract | Elemental carbon exists in different structural forms including graphite, diamond, fullerenes, and amorphous carbon. In nature, these materials are produced through abiotic chemical processes under high temperature and pressure but are considered generally inaccessible to biochemical synthesis or breakdown. Here, we identified and characterized elemental carbon isolated from consortia of anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB), which together carry out the anaerobic oxidation of methane (AOM). Two different AOM consortia, ANME-1a/HotSeep-1 and ANME-2a/c/Seep-SRB, produce a black material with similar characteristics to disordered graphite and amorphous carbon. Stable isotope probing studies revealed that the carbon is microbially generated during AOM. In addition, we found that select methanogens also produce amorphous carbon with similar characteristics to the carbon from AOM consortia. Biogenic amorphous carbon may serve as a conductive element to facilitate electron transfer, or redox active functional groups associated with the carbon could act as electron donors and acceptors. | en |
| dc.description.sponsorship | This work was supported by the National Science Foundation grant MCB1120346 (R.H.W.). G.W. was supported by the DFG excellence cluster 2077 “The Ocean Floor - Earth’s Uncharted Interface” at MARUM, University of Bremen and the Max Planck Society. Partial funding for R.J.B.’s research activities is provided by the National Science Foundation under grant EAR-1624589. SEM/EDS and XPS analyses were performed at the Surface Analysis Laboratory in the Department of Chemistry at Virginia Tech, which is supported by the National Science Foundation under grant no. CHE-1531834. The mass spectrometry resources are maintained by the Virginia Tech Mass Spectrometry Incubator, a facility operated, in part, through funding by the Fralin Life Science Institute at Virginia Tech and by the Agricultural Experiment Station Hatch Program (CRIS project number VA-135981). | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | https://doi.org/10.1126/sciadv.abg9739 | en |
| dc.identifier.issue | 44 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/106578 | en |
| dc.identifier.volume | 7 | en |
| dc.language.iso | en | en |
| dc.publisher | AAAS | en |
| dc.rights | Creative Commons Attribution-NonCommercial 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | en |
| dc.title | Biogenic formation of amorphous carbon by anaerobic methanotrophs and select methanogens | en |
| dc.title.serial | Science Advances | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |