Confirmation of Sarcocystis jamaicensis Sarcocysts in IFN-γ Gene Knockout Mice Orally Inoculated With Sporocysts From a Red-Tailed Hawk (Buteo jamaicensis)

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dc.contributor.authorDubey, Jitender P.en
dc.contributor.authorCerqueira-Cezar, Camila K.en
dc.contributor.authorMurata, Fernando H. A.en
dc.contributor.authorMowery, J. D.en
dc.contributor.authorScott, D.en
dc.contributor.authorvon Dohlen, Alexa Rosypalen
dc.contributor.authorLindsay, David S.en
dc.date.accessioned2020-03-06T17:57:00Zen
dc.date.available2020-03-06T17:57:00Zen
dc.date.issued2019-02-22en
dc.description.abstractHere, we report confirmation of sarcocysts of Sarcocystis jamaicensis in an experimental intermediate host, IFN-gamma gene knockout (KO) mice orally inoculated sporocysts from its natural definitive host, a red-tailed hawk (Buteo jamaicensis) (RTH). A RTH submitted to the Carolina Raptor Center, Huntersville, North Carolina, was euthanized because it could not be rehabilitated and released. Fully sporulated sporocysts from intestinal scrapings of the RTH were orally fed to 2 laboratory-reared outbred Swiss Webster mice (SW; Mus musculus) and to 2 KO mice. The sporocysts were infective for KO mice but not to SW mice. Both SW mice remained asymptomatic, and neither schizonts nor sarcocysts were found in their tissues when euthanized on day 54 post-inoculation (PI). The KO mice developed neurological signs and were necropsied 38-54 days PI. Schizonts/merozoites were found in both KO mice euthanized and they were confined to the brain. The predominant lesion was meningoencephalitis. Microscopic sarcocysts were found in muscles of both KO mice. When viewed with light microscopy, the sarcocyst wall appeared thin (<1 mu m thick) and smooth. Ultrastructural details of sarcocysts are described.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis work was supported in part by grant number 1505407 from the National Science Foundation Historically Black Colleges and Universities Undergraduate Program to A.R.V.D. and an Internal Research Competition (IRC) grant from the Virginia-Maryland College of Veterinary Medicine to D.S.L. This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). ORISE is managed by Oak Ridge Associated Universities (ORAU) under DOE contract number DE-SC0014664. All opinions expressed in this paper are the authors' and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE. The USDA is an equal opportunity provider and employer.en
dc.description.sponsorshipNational Science Foundation Historically Black Colleges and Universities Undergraduate Program [1505407]; Virginia-Maryland College of Veterinary Medicine; U.S. Department of Energy (DOE)United States Department of Energy (DOE); U.S. Department of Agriculture (USDA)United States Department of Agriculture (USDA); DOEUnited States Department of Energy (DOE) [DE-SC0014664]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1645/18-148en
dc.identifier.eissn1937-2345en
dc.identifier.issn0022-3395en
dc.identifier.issue1en
dc.identifier.pmid30807718en
dc.identifier.urihttp://hdl.handle.net/10919/97234en
dc.identifier.volume105en
dc.language.isoenen
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectSarcocystisen
dc.subjectTransmission Electron Microscopyen
dc.subjectMus musculusen
dc.subjectSarcocystis jamaicensisen
dc.subjectMerozoitesen
dc.subjectSchizontsen
dc.subjectEncephalitisen
dc.subjectRed-Tailed Hawken
dc.subjectButeo jamaicensisen
dc.subjectSporocystsen
dc.titleConfirmation of Sarcocystis jamaicensis Sarcocysts in IFN-γ Gene Knockout Mice Orally Inoculated With Sporocysts From a Red-Tailed Hawk (Buteo jamaicensis)en
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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