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A novel frequency analysis method for assessing K(ir)2.1 and Na (v)1.5 currents.

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dc.contributor.authorRigby, J. R.en
dc.contributor.authorPoelzing, Stevenen
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.contributor.departmentFralin Biomedical Research Instituteen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2017-02-03T15:13:58Zen
dc.date.available2017-02-03T15:13:58Zen
dc.date.issued2012-04en
dc.description.abstractVoltage clamping is an important tool for measuring individual currents from an electrically active cell. However, it is difficult to isolate individual currents without pharmacological or voltage inhibition. Herein, we present a technique that involves inserting a noise function into a standard voltage step protocol, which allows one to characterize the unique frequency response of an ion channel at different step potentials. Specifically, we compute the fast Fourier transform for a family of current traces at different step potentials for the inward rectifying potassium channel, K(ir)2.1, and the channel encoding the cardiac fast sodium current, Na(v)1.5. Each individual frequency magnitude, as a function of voltage step, is correlated to the peak current produced by each channel. The correlation coefficient vs. frequency relationship reveals that these two channels are associated with some unique frequencies with high absolute correlation. The individual IV relationship can then be recreated using only the unique frequencies with magnitudes of high absolute correlation. Thus, this study demonstrates that ion channels may exhibit unique frequency responses.en
dc.description.versionPublished versionen
dc.format.extent946 - 954 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1007/s10439-011-0460-9en
dc.identifier.eissn1573-9686en
dc.identifier.issue4en
dc.identifier.urihttp://hdl.handle.net/10919/74922en
dc.identifier.volume40en
dc.language.isoenen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/22052157en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectDielectric Spectroscopyen
dc.subjectHEK293 Cellsen
dc.subjectHumansen
dc.subjectIon Transporten
dc.subjectMembrane Potentialsen
dc.subjectNAV1.5 Voltage-Gated Sodium Channelen
dc.subjectPotassium Channels, Inwardly Rectifyingen
dc.subjectSodium Channelsen
dc.titleA novel frequency analysis method for assessing K(ir)2.1 and Na (v)1.5 currents.en
dc.title.serialAnnals of Biomedical Engineeringen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherResearch Support, N.I.H., Extramuralen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Virginia Tech Carilion Research Instituteen

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