The enzymatic solubilization of crystalline cellulose

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dc.contributor.authorFlora, Robert Montgomeryen
dc.contributor.committeechairKing, Kendall W.en
dc.contributor.committeememberEngel, R. W.en
dc.contributor.departmentBiochemistryen
dc.date.accessioned2014-03-14T21:23:42Zen
dc.date.adate2009-12-23en
dc.date.available2014-03-14T21:23:42Zen
dc.date.issued1964-07-05en
dc.date.rdate2009-12-23en
dc.date.sdate2009-12-23en
dc.description.abstractA crude cellulolytic enzyme prepq.ration derived from Trichoderma viride was capable of solubilizing native crystalline forms of cellulose. Enzyme activity ("hydrocellulase") was determined by measuring the decrease in turbidity of the assay reaction mixture which contained a suspended hydrocellulose substrate. Preliminary studies of "hydrocellulase" showed that under assay conditions maximum activity was obtained at pH 4.7 to 4.8 and at 40° for 3 hours. The activity was relatively stable for a three hour period between pH 4.0 and 7.0 and at temperatures up to 40°. Cellobiose was several times more inhibitory than glucose. Methylcellulose was very inhibitory. Sulfhydryl compounds stimulated activity of the crude preparation. EDTA was without effect.en
dc.description.degreePh. D.en
dc.format.extent99 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-12232009-020727en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-12232009-020727/en
dc.identifier.urihttp://hdl.handle.net/10919/40481en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1964.F566.pdfen
dc.relation.isformatofOCLC# 07595020en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1964.F566en
dc.subject.lcshCelluloseen
dc.subject.lcshEnzymesen
dc.titleThe enzymatic solubilization of crystalline celluloseen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistryen
thesis.degree.grantorVirginia Polytechnic Instituteen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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