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Wearable adjunct ozone and antibiotic therapy system for treatment of Gram-negative dermal bacterial infection

dc.contributor.authorRoth, Alexanderen
dc.contributor.authorMaruthamuthu, Murali Kannanen
dc.contributor.authorNejati, Sinaen
dc.contributor.authorKrishnakumar, Akshayen
dc.contributor.authorSelvamani, Vidhyaen
dc.contributor.authorSedaghat, Sotoudehen
dc.contributor.authorNguyen, Julianeen
dc.contributor.authorSeleem, Mohamed N.en
dc.contributor.authorRahimi, Rahimen
dc.date.accessioned2022-10-17T12:51:25Zen
dc.date.available2022-10-17T12:51:25Zen
dc.date.issued2022-08-17en
dc.description.abstractThe problematic combination of a rising prevalence of skin and soft tissue infections and the growing rate of life-threatening antibiotic resistant infections presents an urgent, unmet need for the healthcare industry. These evolutionary resistances originate from mutations in the bacterial cell walls which prevent effective diffusion of antibiotics. Gram-negative bacteria are of special consideration due to the natural resistance to many common antibiotics due to the unique bilayer structure of the cell wall. The system developed here provides one solution to this problem through a wearable therapy that delivers and utilizes gaseous ozone as an adjunct therapy with topical antibiotics through a novel dressing with drug-eluting nanofibers (NFs). This technology drastically increases the sensitivity of Gram-negative bacteria to common antibiotics by using oxidative ozone to bypass resistances created by the bacterial cell wall. To enable simple and effective application of adjunct therapy, ozone delivery and topical antibiotics have been integrated into a single application patch. The drug delivery NFs are generated via electrospinning in a fast-dissolve PVA mat without inducing decreasing gas permeability of the dressing. A systematic study found ozone generation at 4 mg/h provided optimal ozone levels for high antimicrobial performance with minimal cytotoxicity. This ozone treatment was used with adjunct therapy delivered by the system in vitro. Results showed complete eradication of Gram-negative bacteria with ozone and antibiotics typically used only for Gram-positive bacteria, which showed the strength of ozone as an enabling adjunct treatment option to sensitize bacteria strains to otherwise ineffective antibiotics. Furthermore, the treatment is shown through biocompatibility testing to exhibit no cytotoxic effect on human fibroblast cells.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41598-022-17495-3en
dc.identifier.issn2045-2322en
dc.identifier.issue1en
dc.identifier.other13927en
dc.identifier.pmid35977975en
dc.identifier.urihttp://hdl.handle.net/10919/112178en
dc.identifier.volume12en
dc.language.isoenen
dc.publisherNature Portfolioen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsilver nanoparticlesen
dc.subjectmolecular-weighten
dc.subjectfiber matsen
dc.subjectantibacterialen
dc.subjectvancomycinen
dc.subjectpatchen
dc.subjecthydrolysisen
dc.subjectresistanceen
dc.subjectdeliveryen
dc.subjectreleaseen
dc.titleWearable adjunct ozone and antibiotic therapy system for treatment of Gram-negative dermal bacterial infectionen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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