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Bacterial chemotaxis-enabled autonomous sorting of nanoparticles of comparable sizes

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dc.contributor.authorSuh, SeungBeumen
dc.contributor.authorTraore, Mahama Azizen
dc.contributor.authorBehkam, Baharehen
dc.contributor.departmentMechanical Engineeringen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2017-05-01T06:42:18Zen
dc.date.available2017-05-01T06:42:18Zen
dc.date.issued2016-02-18en
dc.description.abstractHigh throughput sorting of micro/nanoparticles of similar sizes is of significant interest in many biological and chemical applications. In this work, we report a simple and cost-effective sorting technique for separation of similarly-sized particles of dissimilar surface properties within a diffusion-based microfluidic platform using chemotaxis in Escherichia coli bacteria. Differences in surface chemistry of two groups of similarly-sized nanoparticles in a mixture were exploited to selectively assemble one particle group onto motile E. coli, through either specific or non-specific adhesion, and separate them from the remaining particle group via chemotaxis of the attached bacteria. To enable optimal operation of the sorting platform, the chemotaxis behavior of E. coli bacteria in response to casamino acids, the chemoeffector of choice was first characterized. The chemical concentration gradient range within which the bacteria exhibit a positive chemotactic response was found to be within 0.25 × 10−7–1.0 × 10−3 g ml−1 mm−1. We demonstrate that at the optimum concentration gradient of 5.0 × 10−4 g ml−1 mm−1, a sorting efficiency of up to 81% at a throughput of 2.4 × 105 particles per min can be achieved. Sensitivity of the sorting efficiency to the adhesion mechanism and particle size in the range of 320–1040 nm was investigated.en
dc.format.extent1254-1260en
dc.format.mimetypeapplication/pdfen
dc.identifierc6lc00059b.pdfen
dc.identifierc6lc00059b1.pdfen
dc.identifier.doihttps://doi.org/10.1039/c6lc00059ben
dc.identifier.eissn1473-0189en
dc.identifier.issn1473-0197en
dc.identifier.issue7en
dc.identifier.urihttp://hdl.handle.net/10919/77561en
dc.identifier.volume16en
dc.language.isoen_USen
dc.publisherRoyal Society of Chemistryen
dc.relation.ispartofRoyal Society of Chemistry Gold Open Access - 2016en
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.holderSuh, SeungBeumen
dc.rights.holderTraore, Mahama A.en
dc.rights.holderBehkam, Baharehen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.titleBacterial chemotaxis-enabled autonomous sorting of nanoparticles of comparable sizesen
dc.title.serialLab on a Chipen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeDataseten

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