Identification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infection

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dc.contributor.authorDiaz, Joyce Anne R.en
dc.contributor.authorKim, Woo H.en
dc.contributor.authorFernandez, Cherry P.en
dc.contributor.authorJeong, Jipseolen
dc.contributor.authorAfrin, Fahmidaen
dc.contributor.authorLillehoj, Hyun S.en
dc.contributor.authorKim, Suken
dc.contributor.authorKim, Sungwonen
dc.contributor.authorDalloul, Rami A.en
dc.contributor.authorMin, Wongien
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2020-04-21T14:50:30Zen
dc.date.available2020-04-21T14:50:30Zen
dc.date.issued2016-08en
dc.description.abstractInterleukin (IL)-17D is a proinfiammatory cytokine with currently largely unknown biological functions. Here we provide the description of the sequence, bioactivity, and mRNA expression profile of duck IL-17D homologue. A full-length duck IL-17D (duIL-17D) cDNA with a 624-bp coding region was identified from the large intestine. dull-17D shares approximately 94.7% identity with its chicken counterpart, which is also identified in this work. duIL-17D exhibits 62.6-68.4% and 52.1-53.1% identity with mammalian and piscine homologues. Recombinant duIL-17D promoted the expression of proinfiammatory cytokines such as IL-6, IL-8, and IL-1 beta in duck embryo fibroblast cells. Very low levels of dull-17D transcript were observed in healthy lymphoid tissues, including bursa, thymus, and spleen, while duIL-17D expression was relatively high in the heart. The dull-17D expression profiles were examined in mitogen-stimulated splenic lymphocytes, as well as tissues affected by Riemerella anatipestifer infection. The levels of duIL-17D were mostly upregulated in mitogen-activated splenic lymphocytes but downregulated in the liver and spleen of R. anatipestifer-infected ducks. These results provide new insights into the roles of IL-17D in host protective immune responses to Riemerella infection, which can therefore lead to further studies of its biological functions in different disease models of ducks and other avian species.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A02059953).en
dc.description.sponsorshipBasic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2015R1D1A1A02059953]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.dci.2016.04.002en
dc.identifier.eissn1879-0089en
dc.identifier.issn0145-305Xen
dc.identifier.pmid27060655en
dc.identifier.urihttp://hdl.handle.net/10919/97855en
dc.identifier.volume61en
dc.language.isoenen
dc.rightsCC0 1.0 Universalen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectDucksen
dc.subjectInterleukin-17Den
dc.subjectRiemerella infectionen
dc.subjectAvian IL-17Den
dc.titleIdentification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infectionen
dc.title.serialDevelopmental and Comparative Immunologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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