Plasma lipids and lipoproteins in male subjects under controlled conditions of high cholesterol feeding

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Virginia Polytechnic Institute and State University


Twenty-three university male students: were fed mixed food diets, containing either 400 mg or 1400 mg cholesterol/day under controlled dietary conditions for 4 consecutive weeks. One group received a diet containing 400 mg cholesterol/day, which was contributed by non-egg food sources. The other group received a similar diet with the daily addition of 1000 mg cholesterol as 4 whole eggs. Diets were adjusted so differences in total protein, carbohydrate, fat, and fatty acid composition were minimal between the two dietary treatments. Plasma total cholesterol, triglycerides, and HDL, LDL, and VLDL cholesterol levels were measured at the beginning, weekly throughout the experimental period, and 1 week after completion of the study. No significant changes in any of the measured parameters were observed as a result of the high cholesterol diet. Comparisons of BDL, LDL, and VLDL cholesterol concentrations expressed as percentages of plasma total cholesterol showed that lipoprotein cholesterol distribution remained unchanged within and between treatments throughout the study. Relative concentrations of plasma lipoproteins at the beginning or the end of dietary treatment were similar in subjects receiving 400 mg or 1400 mg cholesterol/day. No detectable ApoE was observed by SDS-PAGE in HDL₂ or HDL₃ samples from either treatment group.