Cowpea chlorotic mottle bromovirus replication proteins support template-selective RNA replication in Saccharomyces cerevisiae

dc.contributor.authorSibert, Bryan S.en
dc.contributor.authorNavine, Amanda K.en
dc.contributor.authorPennington, Janiceen
dc.contributor.authorWang, Xiaofengen
dc.contributor.authorAhlquist, Paulen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-05-30T14:34:27Zen
dc.date.available2019-05-30T14:34:27Zen
dc.date.issued2018-12-26en
dc.description.abstractPositive-strand RNA viruses generally assemble RNA replication complexes on rearranged host membranes. Alphaviruses, other members of the alpha-like virus superfamily, and many other positive-strand RNA viruses invaginate host membrane into vesicular RNA replication compartments, known as spherules, whose interior is connected to the cytoplasm. Brome mosaic virus (BMV) and its close relative, cowpea chlorotic mottle virus (CCMV), form spherules along the endoplasmic reticulum. BMV spherule formation and RNA replication can be fully reconstituted in S. cerevisiae, enabling many studies identifying host factors and viral interactions essential for these processes. To better define and understand the conserved, core pathways of bromovirus RNA replication, we tested the ability of CCMV to similarly support spherule formation and RNA replication in yeast. Paralleling BMV, we found that CCMV RNA replication protein 1a was the only viral factor necessary to induce spherule membrane rearrangements and to recruit the viral 2a polymerase (2a(pol)) to the endoplasmic reticulum. CCMV 1a and 2a(pol) also replicated CCMV and BMV genomic RNA2, demonstrating core functionality of CCMV 1a and 2a(pol) in yeast. However, while BMV and CCMV 1a/2a(pol) strongly replicate each others' genomic RNA3 in plants, neither supported detectable CCMV RNA3 replication in yeast. Moreover, in contrast to plant cells, in yeast CCMV 1a/2a(pol) supported only limited replication of BMV RNA3 (<5% of that by BMV 1a/2a(pol)). In keeping with this, we found that in yeast CCMV 1a was significantly impaired in recruiting BMV or CCMV RNA3 to the replication complex. Overall, we show that many 1a and 2a(pol) functions essential for replication complex assembly, and their ability to be reconstituted in yeast, are conserved between BMV and CCMV. However, restrictions of CCMV RNA replication in yeast reveal previously unknown 1a-linked, RNA-selective host contributions to the essential early process of recruiting viral RNA templates to the replication complex.en
dc.description.notesP.A. is an investigator of the Howard Hughes Medical Institute and the Morgridge Institute for Research, and gratefully acknowledges support from these institutes, the NIH, and the John W. and Jeanne M. Rowe Virology Venture Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.description.sponsorshipNIH; John W. and Jeanne M. Rowe Virology Venture Funden
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0208743en
dc.identifier.eissn1932-6203en
dc.identifier.issue12en
dc.identifier.othere0208743en
dc.identifier.pmid30586378en
dc.identifier.urihttp://hdl.handle.net/10919/89641en
dc.identifier.volume13en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleCowpea chlorotic mottle bromovirus replication proteins support template-selective RNA replication in Saccharomyces cerevisiaeen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen
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