Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins

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dc.contributor.authorHelliwell, Emily E.en
dc.contributor.authorVega-Arreguin, Julioen
dc.contributor.authorShi, Zien
dc.contributor.authorBailey, Bryanen
dc.contributor.authorXiao, Shunyuanen
dc.contributor.authorMaximova, Siela N.en
dc.contributor.authorTyler, Brett M.en
dc.contributor.authorGuiltinan, Mark J.en
dc.contributor.departmentFralin Life Sciences Instituteen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-04-23T13:09:48Zen
dc.date.available2020-04-23T13:09:48Zen
dc.date.issued2016-03en
dc.description.abstractThe internalization of some oomycete and fungal pathogen effectors into host plant cells has been reported to be blocked by proteins that bind to the effectors' cell entry receptor, phosphatidylinositol-3-phosphate (PI3P). This finding suggested a novel strategy for disease control by engineering plants to secrete PI3P-binding proteins. In this study, we tested this strategy using the chocolate tree Theobroma cacao. Transient expression and secretion of four different PI3P-binding proteins in detached leaves of T.cacao greatly reduced infection by two oomycete pathogens, Phytophthora tropicalis and Phytophthora palmivora, which cause black pod disease. Lesion size and pathogen growth were reduced by up to 85%. Resistance was not conferred by proteins lacking a secretory leader, by proteins with mutations in their PI3P-binding site, or by a secreted PI4P-binding protein. Stably transformed, transgenic T.cacao plants expressing two different PI3P-binding proteins showed substantially enhanced resistance to both P.tropicalis and P.palmivora, as well as to the fungal pathogen Colletotrichum theobromicola. These results demonstrate that secretion of PI3P-binding proteins is an effective way to increase disease resistance in T.cacao, and potentially in other plants, against a broad spectrum of pathogens.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesWe would like to thank Lena Sheaffer and Sharon Pishak for the technical assistance in maintenance of our cacao tissue culture and transformation pipelines, German Sandoya for assistance with statistical analyses, Daniel McClosky for assistance with staining and microscopy procedures, Dylan Storey for advice on distinguishing P. tropicalis from P. capsici, and Brent Kronmiller for bioinformatics assistance. We are also grateful to Andrew Fister and Yufan Zhang for valuable comments throughout this project. This work was supported in part by The Pennsylvania State University, College of Agricultural Sciences, The Huck Institutes of Life Sciences, the American Research Institute Penn State Endowed Program in the Molecular Biology of Cacao and grants from the National Science Foundation BREAD Program (IOS-0965353) to BT, MG, SM and SX, and to BT from the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number (2011-68004-30104).en
dc.description.sponsorshipPennsylvania State University, College of Agricultural Sciences; Huck Institutes of Life Sciences; American Research Institute Penn State Endowed Program in the Molecular Biology of Cacao; National Science Foundation BREAD Program [IOS-0965353]; National Institute of Food and Agriculture, U.S. Department of AgricultureUnited States Department of Agriculture (USDA) [2011-68004-30104]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1111/pbi.12436en
dc.identifier.eissn1467-7652en
dc.identifier.issn1467-7644en
dc.identifier.issue3en
dc.identifier.pmid26214158en
dc.identifier.urihttp://hdl.handle.net/10919/97896en
dc.identifier.volume14en
dc.language.isoenen
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectTheobroma cacaoen
dc.subjectdisease resistanceen
dc.subjectphosphatidylinositol-3-phosphate-binding proteinen
dc.subjecteffectorsen
dc.subjectoomycetesen
dc.subjectfungien
dc.titleEnhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteinsen
dc.title.serialPlant Biotechnology Journalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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