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Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos

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dc.contributor.authorPark, Chi-Hunen
dc.contributor.authorJeoung, Young-Heeen
dc.contributor.authorUh, Kyungjunen
dc.contributor.authorPark, Ki-Eunen
dc.contributor.authorBridge, Jessicaen
dc.contributor.authorPowell, Anne M.en
dc.contributor.authorLi, Jieen
dc.contributor.authorPence, Laramieen
dc.contributor.authorZhang, Luhuien
dc.contributor.authorLiu, Tianbinen
dc.contributor.authorSun, Hai-Xien
dc.contributor.authorGu, Yingen
dc.contributor.authorShen, Yueen
dc.contributor.authorWu, Junen
dc.contributor.authorBelmonte, Juan-Carlos Izpisuaen
dc.contributor.authorTelugu, Bhanu P.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2021-03-03T18:23:17Zen
dc.date.available2021-03-03T18:23:17Zen
dc.date.issued2021-01-12en
dc.description.abstractMost of our current knowledge regarding early lineage specification and embryo-derived stem cells comes from studies in rodent models. However, key gaps remain in our understanding of these developmental processes from nonrodent species. Here, we report the detailed characterization of pig extraembryonic endoderm (pXEN) cells, which can be reliably and reproducibly generated from primitive endoderm (PrE) of blastocyst. Highly expandable pXEN cells express canonical PrE markers and transcriptionally resemble rodent XENs. The pXEN cells contribute both to extraembryonic tissues including visceral yolk sac as well as embryonic gut when injected into host blastocysts, and generate live offspring when used as a nuclear donor in somatic cell nuclear transfer (SCNT). The pXEN cell lines provide a novel model for studying lineage segregation, as well as a source for genome editing in livestock.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis research was supported by AFRI Grants#2015-67015-22845 and 2018-67015-27575 from the USDA National Institute of Food and Agriculture, and 1 R01 HD092304-01A1 from NICHD, NIH to B.T.; Guangdong Provincial Key Laboratory of Genome Read and Write (No. 2017B030301011), Guangdong Provincial Academician Workstation of BGI Synthetic Genomics (No. 2017B090904014), Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics (DRC-SZ[2016]884) and Shenzhen Peacock Plan (No.. KQTD20150330171505310)en
dc.description.sponsorshipAFRI from the USDA National Institute of Food and Agriculture [2015-67015-22845, 2018-67015-27575]; NICHD, NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) [1 R01 HD092304-01A1]; Guangdong Provincial Key Laboratory of Genome Read and Write [2017B030301011]; Guangdong Provincial Academician Workstation of BGI Synthetic Genomics [2017B090904014]; Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics [DRC-SZ[2016]884]; Shenzhen Peacock Plan [KQTD20150330171505310]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.stemcr.2020.11.011en
dc.identifier.issn2213-6711en
dc.identifier.issue1en
dc.identifier.pmid33338433en
dc.identifier.urihttp://hdl.handle.net/10919/102587en
dc.identifier.volume16en
dc.language.isoenen
dc.rightsPublic Domainen
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/en
dc.titleExtraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryosen
dc.title.serialStem Cell Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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