Distinguishing between overdrive excited and suppressed ventricular beats in guinea pig ventricular myocardium

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dc.contributor.authorGreer-Short, Amara D.en
dc.contributor.authorPoelzing, Stevenen
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.contributor.departmentFralin Biomedical Research Instituteen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2017-02-03T15:16:04Zen
dc.date.available2017-02-03T15:16:04Zen
dc.date.issued2015-02-18en
dc.description.abstractRapid ventricular pacing rates induces two types of beats following pacing cessation: recovery cycle length (RCL) prolongation (overdrive suppression) and RCL shortening (overdrive excitation). The goals of this study were to compare common experimental protocols for studying triggered activity in whole-heart preparations and differentiate between recovery beats using a new methodology. Post-pacing recovery beat cycle length (RCL) and QRS were normalized to pre-paced R-R and QRS intervals and analyzed using a K-means clustering algorithm. Control hearts only produced suppressed beats: RCL ratio increased with rapid pacing (25 +/- 4.0%, n = 10) without changing QRS duration. Rapid pacing during hypercalcemia + hypothermia (5.5 mM and 34 degrees C) produced significantly earlier excited beats (53 +/- 14%, n = 5) with wider QRS durations (58 +/- 6.3%, n = 5) than suppressed beats. Digoxin + hypothermia (0.75 mu M) produced the most excited beats with significantly earlier RCL (44 +/- 3.2%, n = 6) and wider QRS (60 +/- 3.1%, n = 6) ratios relative to suppressed beats. Increasing pacing further shortened RCL (30 +/- 7.8%, n = 6). In a prospective study, TTX (100 nM) increased RCL ratio (15 +/- 6.0%, n = 10) without changing the QRS duration of excited beats. The algorithm was compared to a cross-correlation analysis with 93% sensitivity and 94% specificity. This ECG based algorithm distinguishes between triggered and automatic activity.en
dc.description.versionPublished versionen
dc.format.extent10 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fphys.2015.00014en
dc.identifier.issn1664-042Xen
dc.identifier.urihttp://hdl.handle.net/10919/74924en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352932100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPhysiologyen
dc.subjectautomaticityen
dc.subjecttriggered activityen
dc.subjectoverdrive excitationen
dc.subjectoverdrive suppressionen
dc.subjecthypothermiaen
dc.subjectdigoxinen
dc.subjecthypercalcemiaen
dc.subjectCARDIAC PURKINJE-FIBERSen
dc.subjectDELAYED AFTERDEPOLARIZATIONSen
dc.subjectTRANSMEMBRANE POTENTIALSen
dc.subjectCALCIUM-CONCENTRATIONen
dc.subjectTRIGGERED ACTIVITYen
dc.subjectCANINE HEARTen
dc.subjectNA+ CHANNELSen
dc.subjectWHOLE HEARTen
dc.subjectIN-VIVOen
dc.subjectARRHYTHMIASen
dc.titleDistinguishing between overdrive excited and suppressed ventricular beats in guinea pig ventricular myocardiumen
dc.title.serialFrontiers in Physiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Virginia Tech Carilion Research Instituteen

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