Carbonic anhydrase from Methanosarcina thermophila: proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolism

dc.contributor.authorAlber, Birgit E.en
dc.contributor.committeechairFerry, James G.en
dc.contributor.committeememberDean, Dennis R.en
dc.contributor.committeememberJohnson, John L.en
dc.contributor.committeememberGregory, Eugene M.en
dc.contributor.committeememberNiehaus, Walter G. Jr.en
dc.contributor.departmentBiochemistry and Anaerobic Microbiologyen
dc.date.accessioned2014-03-14T21:14:34Zen
dc.date.adate2008-06-06en
dc.date.available2014-03-14T21:14:34Zen
dc.date.issued1995-06-09en
dc.date.rdate2008-06-06en
dc.date.sdate2008-06-06en
dc.description.abstractCarbonic anhydrase (CA) from acetate-grown Methanosarcina thermophila strain TM-1 was purified> 10,OOO-fold (22% recovery) to apparent homogeneity and a specific activity of 4,900 units mg⁻¹.The gene encoding this CA was isolated fronl a partial genomic library on a 12-kb fragment and sequenced. Comparison of the deduced anlino acid sequence with the N-terminaI sequence of the purified protein shows that the gene encodes an additional 34 N-terminal residues with properties characteristic of signal peptides in secretory proteins. The deduced amino acid sequence has no significant identity to any known CAs, but has, among others, 35% sequence identity to the first 197 deduced N-terminal amino acids of a proposed CO₂-concentrating-mechanism protein from <i>Synechococcus</i> sp. strain PCC7942.en
dc.description.degreePh. D.en
dc.format.extentxiii, 153 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06062008-171625en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06062008-171625/en
dc.identifier.urihttp://hdl.handle.net/10919/38486en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1995.A438.pdfen
dc.relation.isformatofOCLC# 34489092en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectcarbonic anhydraseen
dc.subjectCAen
dc.subject.lccLD5655.V856 1995.A438en
dc.titleCarbonic anhydrase from Methanosarcina thermophila: proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolismen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Anaerobic Microbiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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