The gamma interferon knockout mouse model for Sarcocystis neurona: Comparison of infectivity of sporocysts and merozoites and routes of inoculation

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dc.contributor.authorDubey, Jitender P.en
dc.contributor.authorLindsay, David S.en
dc.contributor.authorKwok, O. C. H.en
dc.contributor.authorShen, S. K.en
dc.date.accessed2014-06-16en
dc.date.accessioned2014-06-20T14:13:14Zen
dc.date.available2014-06-20T14:13:14Zen
dc.date.issued2001-10en
dc.description.abstractThe dose-related infectivity of Sarcocystis neurona sporocysts and merozoites. of 2 recent isolates of S. neurona was compared in gamma interferon knockout (KO) mice. Tenfold dilutions of sporocysts or merozoites were bioassayed in mice, cell culture, or both. All 8 mice, fed 1,000 sporocysts, developed neurological signs with demonstrable S. neurona in their tissues. Of 24 mice fed low numbers of sporocysts. (100, 10, 1), 18 became ill by 4 wk postinoculation, and S. neurona was demonstrated in their brains; antibodies (S. neurona agglutination test) to S. neurona and S. neurona parasites were not found in tissues of the 6 mice that were fed sporocysts and survived for >39 days. One thousand culture-derived merozoites of these 2 isolates were pathogenic to all 8 mice inoculated subcutaneously (s.c.). Of the 24 mice inoculated s.c. with merozoites. numbering 100, 10, or 1, only 3 mice had demonstrable S. neurona infection; antibodies to S. neurona were not found in the 21 mice that had no demonstrable organisms. As few as 10 merozoites. were infective for cell cultures. These results demonstrate that at least 1,000 merozoites are needed to cause disease in KO mice. Sarcocystis neurona sporocysts were infective to mice by the s.c. route.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationJ. P. Dubey, D. S. Lindsay, O. C H. Kwok, and S. K. Shen (2001). "The Gamma Interferon Knockout Mouse Model for Sarcocystis neurona: Comparison of Infectivity of Sporocysts and Merozoites and Routes of Inoculation," Journal of Parasitology, Vol. 87, No. 5, pp. 1171-1173. doi: http://dx.doi.org/10.1645/0022-3395(2001)087[1171:TGIKMM]2.0.CO;2en
dc.identifier.doihttps://doi.org/10.1645/0022-3395(2001)087[1171:tgikmm]2.0.co;2en
dc.identifier.issn0022-3395en
dc.identifier.urihttp://hdl.handle.net/10919/49050en
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/0022-3395%282001%29087%5B1171%3ATGIKMM%5D2.0.CO%3B2en
dc.language.isoenen
dc.publisherAmerican Society of Parasitologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectequine protozoal myeloencephalitisen
dc.subjectopossum didelphis-virginianaen
dc.subjectn-spen
dc.subjectprotozoaen
dc.subjectfalcatulaen
dc.subjectmiceen
dc.subjectparasitologyen
dc.titleThe gamma interferon knockout mouse model for Sarcocystis neurona: Comparison of infectivity of sporocysts and merozoites and routes of inoculationen
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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