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Assessing the Enzymatic Hydrolysis of Salmon Frame Proteins through Different By-Product/Water Ratios and pH Regimes

dc.contributor.authorValencia, Pedroen
dc.contributor.authorValdivia, Silvanaen
dc.contributor.authorNuñez, Suleivysen
dc.contributor.authorOvissipour, Rezaen
dc.contributor.authorPinto, Marleneen
dc.contributor.authorRamirez, Cristianen
dc.contributor.authorPerez, Alvaroen
dc.contributor.authorRuz, Manuelen
dc.contributor.authorGarcia, Paulaen
dc.contributor.authorJimenez, Paulaen
dc.contributor.authorAlmonacid, Sergioen
dc.date.accessioned2021-12-09T20:00:19Zen
dc.date.available2021-12-09T20:00:19Zen
dc.date.issued2021-12-08en
dc.date.updated2021-12-09T14:32:43Zen
dc.description.abstractThe enzymatic hydrolysis of fish by-product proteins is traditionally carried out by mixing ground by-products with water. In addition, pH control is used to avoid pH drops. Higher costs are involved due to the use of pH control systems and the consequent energy cost in the drying stage. This work aimed to evaluate the effect of these conditions on the hydrolysis of salmon frame (SF) proteins, including the SF hydrolysis without added water. SF hydrolysis by subtilisin at 50, 75, and 100% SF under different pH regimes were evaluated by released &alpha;-amino (&alpha;-NH) groups, total nitrogen, degree of hydrolysis, and estimated peptide chain length (<i>PCL</i>) at 55 &deg;C. The concentration of released &alpha;-NH groups was higher in the conditions with less added water. However, the nitrogen recovery decreased from 50 to 24% at 50 and 100% SF, respectively. Changing the SF/water ratio had a more significant effect than changing the pH regime. Estimated <i>PCL</i> changed from 5&ndash;7 to 7&ndash;9 at 50 and 100% SF, respectively. The operating conditions affected the hydrolysis performance and the molecular characteristics of the hydrolysate.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationValencia, P.; Valdivia, S.; Nuñez, S.; Ovissipour, R.; Pinto, M.; Ramirez, C.; Perez, A.; Ruz, M.; Garcia, P.; Jimenez, P.; Almonacid, S. Assessing the Enzymatic Hydrolysis of Salmon Frame Proteins through Different By-Product/Water Ratios and pH Regimes. Foods 2021, 10, 3045.en
dc.identifier.doihttps://doi.org/10.3390/foods10123045en
dc.identifier.urihttp://hdl.handle.net/10919/106910en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectprotein hydrolysisen
dc.subjectenzymatic hydrolysisen
dc.subjectfish by-producten
dc.subjecthigh by-product concentrationen
dc.titleAssessing the Enzymatic Hydrolysis of Salmon Frame Proteins through Different By-Product/Water Ratios and pH Regimesen
dc.title.serialFoodsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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