Particle-mediated Histotripsy for the Targeted Treatment of Intraluminal Biofilms in Catheter-based Medical Devices

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dc.contributor.authorChilders, Christopheren
dc.contributor.authorEdsall, Connoren
dc.contributor.authorMehochko, Isabelleen
dc.contributor.authorMustafa, Waleeden
dc.contributor.authorYuksel Durmaz, Yaseminen
dc.contributor.authorKlibanov, Alexander L.en
dc.contributor.authorRao, Jayasimhaen
dc.contributor.authorVlaisavljevich, Elien
dc.date.accessioned2022-09-23T19:16:31Zen
dc.date.available2022-09-23T19:16:31Zen
dc.date.issued2022-08-09en
dc.date.updated2022-09-23T18:36:26Zen
dc.description.abstractObjective: This paper is an initial work towards developing particle-mediated histotripsy (PMH) as a novel method of treating catheter-based medical device (CBMD) intraluminal biofilms. Impact Statement: CBMDs commonly become infected with bacterial biofilms leading to medical device failure, infection, and adverse patient outcomes. Introduction: Histotripsy is a noninvasive focused ultrasound ablation method that was recently proposed as a novel method to remove intraluminal biofilms. Here, we explore the potential of combining histotripsy with acoustically active particles to develop a PMH approach that can noninvasively remove biofilms without the need for high acoustic pressures or real-time image guidance for targeting. Methods: Histotripsy cavitation thresholds in catheters containing either gas-filled microbubbles (MBs) or fluid-filled nanocones (NCs) were determined. The ability of these particles to sustain cavitation over multiple ultrasound pulses was tested after a series of histotripsy exposures. Next, the ability of PMH to generate selective intraluminal cavitation without generating extraluminal cavitation was tested. Finally, the biofilm ablation and bactericidal capabilities of PMH were tested using both MBs and NCs. Results: PMH significantly reduced the histotripsy cavitation threshold, allowing for selective luminal cavitation for both MBs and NCs. Results further showed PMH successfully removed intraluminal biofilms in Tygon catheters. Finally, results from bactericidal experiments showed minimal reduction in bacteria viability. Conclusion: The results of this study demonstrate the potential for PMH to provide a new modality for removing bacterial biofilms from CBMDs and suggest that additional work is warranted to develop histotripsy and PMH for treatment of CBMD intraluminal biofilms.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.34133/2022/9826279en
dc.identifier.eissn2765-8031en
dc.identifier.issn2765-8031en
dc.identifier.orcidVlaisavljevich, Eli [0000-0002-4097-6257]en
dc.identifier.orcidRao, Jayasimha [0000-0002-0133-2862]en
dc.identifier.urihttp://hdl.handle.net/10919/111988en
dc.identifier.volume2022en
dc.language.isoenen
dc.publisherAmerican Association for the Advancement of Science (AAAS)en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBiomedical Imagingen
dc.subjectBioengineeringen
dc.subjectInfectionen
dc.titleParticle-mediated Histotripsy for the Targeted Treatment of Intraluminal Biofilms in Catheter-based Medical Devicesen
dc.title.serialBME Frontiersen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/Biomedical Engineering and Mechanicsen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicineen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/Internal Medicineen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/Internal Medicine/General IMen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/TEACH Membersen

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