In Situ Real-Time Chemiluminescence Imaging of Reactive Oxygen Species Formation from Cardiomyocytes

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dc.contributor.authorLi, Yunboen
dc.contributor.authorShen, Haiouen
dc.contributor.authorZhu, Hongen
dc.contributor.authorTrush, Michael A.en
dc.contributor.authorJiang, Mingen
dc.contributor.authorWang, Geen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.contributor.departmentVirginia-Maryland College of Veterinary Medicineen
dc.date.accessioned2017-09-18T10:02:39Zen
dc.date.available2017-09-18T10:02:39Zen
dc.date.issued2009-02-25en
dc.date.updated2017-09-18T10:02:39Zen
dc.description.abstractWe have applied the highly sensitive chemiluminescence (CL) imagingtechnique to investigate the in situ ROS formation in cultured monolayers of rat H9c2 cardiomyocytes. Photon emission was detected via an innovative imaging system after incubation of H9c2 cells in culture with luminol and horseradish peroxidase (HRP), suggesting constitutive formation of ROS by the cardiomyocytes. Addition of benzo(a)pyrene-1,6-quinone(BPQ) to cultured H9c2 cells resulted in a 4-5-fold increase in the formation of ROS, as detected by the CL imaging. Both constitutive and BPQ-stimulated CL responses in cultured H9c2 cells were sustained for up to 1 hour. The CL responses were completely abolished in the presence of superoxide dismutase and catalase, suggesting the primary involvement of superoxide and hydrogen peroxide (). In contrast to BPQ-mediated redox cycling, blockage of mitochondrial electron transport chain by either antimycin A or rotenone exerted marginal effects on the ROS formation by cultured H9c2 cells. Upregulation of cellular antioxidants fordetoxifying both superoxide and by 3-1,2-dithiole-3-thione resulted in marked inhibition of both constitutive and BPQ-augmented ROS formation in cultured H9c2 cells. Taken together, we demonstrate the sensitive detection of ROS by CL imaging in cultured cardiomyocytes.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationYunbo Li, Haiou Shen, Hong Zhu, Michael A. Trush, Ming Jiang, and Ge Wang, “In Situ Real-Time Chemiluminescence Imaging of Reactive Oxygen Species Formation from Cardiomyocytes,” International Journal of Biomedical Imaging, vol. 2008, Article ID 941729, 9 pages, 2008. doi:10.1155/2008/941729en
dc.identifier.doihttps://doi.org/10.1155/2008/941729en
dc.identifier.urihttp://hdl.handle.net/10919/79058en
dc.language.isoenen
dc.publisherHindawien
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderCopyright © 2008 Yunbo Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIn Situ Real-Time Chemiluminescence Imaging of Reactive Oxygen Species Formation from Cardiomyocytesen
dc.title.serialInternational Journal of Biomedical Imagingen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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