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Overcoming Biomass Recalcitrance by Combining Genetically Modified Switchgrass and Cellulose Solvent-Based Lignocellulose Pretreatment

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dc.contributor.authorSathitsuksanoh, Noppadonen
dc.contributor.authorXu, Binen
dc.contributor.authorZhao, Bingyu Y.en
dc.contributor.authorZhang, Y. H. Percivalen
dc.contributor.departmentBiological Systems Engineeringen
dc.contributor.departmentInstitute for Critical Technology and Applied Scienceen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2015-10-01T23:24:33Zen
dc.date.available2015-10-01T23:24:33Zen
dc.date.issued2013-09-27en
dc.description.abstractDecreasing lignin content of plant biomass by genetic engineering is believed to mitigate biomass recalcitrance and improve saccharification efficiency of plant biomass. In this study, we compared two different pretreatment methods (i.e., dilute acid and cellulose solvent) on transgenic plant biomass samples having different lignin contents and investigated biomass saccharification efficiency. Without pretreatment, no correlation was observed between lignin contents of plant biomass and saccharification efficiency. After dilute acid pretreatment, a strong negative correlation between lignin content of plant samples and overall glucose release was observed, wherein the highest overall enzymatic glucan digestibility was 70% for the low-lignin sample. After cellulose solvent- and organic solvent-based lignocellulose fractionation pretreatment, there was no strong correlation between lignin contents and high saccharification efficiencies obtained (i.e., 80-90%). These results suggest that the importance of decreasing lignin content in plant biomass to saccharification was largely dependent on pretreatment choice and conditions.en
dc.description.sponsorshipVirginia Polytechnic and State Universityen
dc.description.sponsorshipVirginia Polytechnic and State University. Institute for Critical Technology and Applied Scienceen
dc.description.sponsorshipVirginia Polytechnic and State University. Department of Biological Systems Engineeringen
dc.description.sponsorshipVirginia Polytechnic Institute and State University. College of Agriculture and Life Sciences. Biodesign and Bioprocessing Research Centeren
dc.description.sponsorshipU.S. Department of Energyen
dc.description.sponsorshipDE-FG02-08ER64629en
dc.format.extent6 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSathitsuksanoh, N., Xu, B., Zhao, B., & Zhang, Y. H. P. (2013). Overcoming Biomass Recalcitrance by Combining Genetically Modified Switchgrass and Cellulose Solvent-Based Lignocellulose Pretreatment. Plos One, 8(9), e73523. doi: 10.1371/journal.pone.0073523en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0073523en
dc.identifier.issn1932-6203en
dc.identifier.issue9en
dc.identifier.urihttp://hdl.handle.net/10919/56677en
dc.identifier.volume8en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectAccessibilityen
dc.subjectBiofuel productionen
dc.subjectCorn stoveren
dc.subjectDilute-acid pretreatmenten
dc.subjectEnzymatic-hydrolysisen
dc.subjectEthanolen
dc.subjectFermentable sugar yieldsen
dc.subjectFractionationen
dc.subjectLignin modificationen
dc.subjectSaccharificationen
dc.titleOvercoming Biomass Recalcitrance by Combining Genetically Modified Switchgrass and Cellulose Solvent-Based Lignocellulose Pretreatmenten
dc.title.serialPloS Oneen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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Scholarly Works, Biological Systems Engineering
Scholarly Works, Institute for Critical Technology and Applied Science (ICTAS)
Scholarly Works, School of Plant and Environmental Sciences
Strategic Growth Area: Economical and Sustainable Materials (ESM)