Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

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dc.contributor.authorSalmanzadeh, Alirezaen
dc.contributor.authorKittur, Harshaen
dc.contributor.authorSano, Michael B.en
dc.contributor.authorRoberts, Paul C.en
dc.contributor.authorSchmelz, Eva M.en
dc.contributor.authorDavalos, Rafael V.en
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.contributor.departmentInstitute for Critical Technology and Applied Scienceen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessed2014-05-09en
dc.date.accessioned2014-05-14T13:35:38Zen
dc.date.available2014-05-14T13:35:38Zen
dc.date.issued2012-06-01en
dc.description.abstractOvarian cancer is the leading cause of death from gynecological malignancies in women. The primary challenge is the detection of the cancer at an early stage, since this drastically increases the survival rate. In this study we investigated the dielectrophoretic responses of progressive stages of mouse ovarian surface epithelial (MOSE) cells, as well as mouse fibroblast and macrophage cell lines, utilizing contactless dielectrophoresis (cDEP). cDEP is a relatively new cell manipulation technique that has addressed some of the challenges of conventional dielectrophoretic methods. To evaluate our microfluidic device performance, we computationally studied the effects of altering various geometrical parameters, such as the size and arrangement of insulating structures, on dielectrophoretic and drag forces. We found that the trapping voltage of MOSE cells increases as the cells progress from a non-tumorigenic, benign cell to a tumorigenic, malignant phenotype. Additionally, all MOSE cells display unique behavior compared to fibroblasts and macrophages, representing normal and inflammatory cells found in the peritoneal fluid. Based on these findings, we predict that cDEP can be utilized for isolation of ovarian cancer cells from peritoneal fluid as an early cancer detection tool. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3699973] Actual pdf downloaded from NCBI.en
dc.description.sponsorshipNational Science Foundation EFRI 0938047en
dc.description.sponsorshipVirginia Tech Institute for Critical Technology and Applied Science (ICTAS)en
dc.description.sponsorshipDiversity Summer Research Program (DSRP)en
dc.description.sponsorshipNIH RO1 CA118846en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSalmanzadeh, A.; Kittur, H.; Sano, M. B.; Roberts, P. C.; Schmelz, E. M.; Davalos, R. V., "Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis," Biomicrofluidics 6, 024104 (2012); http://dx.doi.org/10.1063/1.3699973en
dc.identifier.doihttps://doi.org/10.1063/1.3699973en
dc.identifier.issn1932-1058en
dc.identifier.urihttp://hdl.handle.net/10919/47982en
dc.identifier.urlhttp://scitation.aip.org/content/aip/journal/bmf/6/2/10.1063/1.3699973en
dc.language.isoenen
dc.publisherAmerican Institute of Physicsen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectForce microscopyen
dc.subjectBreast canceren
dc.subjectDielectricsen
dc.subjectStem-cellsen
dc.subjectSeparationen
dc.subjectBlooden
dc.subjectElectrorotationen
dc.subjectBiomarkersen
dc.subjectFrequencyen
dc.subjectMembranesen
dc.subjectBiochemical research methodsen
dc.subjectBiophysicsen
dc.subjectNanoscience & nanotechnologyen
dc.subjectPhysics, fluids & plasmasen
dc.titleDielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresisen
dc.title.serialBiomicrofluidicsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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