The effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formation

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dc.contributor.authorHashimoto, Keien
dc.contributor.authorYamashita, Kimikoen
dc.contributor.authorEnoyoshi, Kanakoen
dc.contributor.authorDahan, Xavieren
dc.contributor.authorTakeuchi, Tatsuen
dc.contributor.authorKori, Hiroshien
dc.contributor.authorGotoh, Marien
dc.contributor.departmentPhysicsen
dc.date.accessioned2020-12-14T15:46:04Zen
dc.date.available2020-12-14T15:46:04Zen
dc.date.issued2020-09en
dc.description.abstractMotile human-skin fibroblasts form macroscopic swirling patterns when grown to confluence on a culture dish. In this paper, we investigate the effect of coating the culture-dish surface with collagen on the resulting pattern, using human-skin fibroblast NB1RGB cells as the model system. The presence of the collagen coating is expected to enhance the adherence of the fibroblasts to the dish surface, and thereby also enhance the traction that the fibroblasts have as they move. We find that, contrary to our initial expectation, the coating does not significantly affect the motility of the fibroblasts. Their eventual number density at confluence is also unaffected. However, the coherence length of cell orientation in the swirling pattern is diminished. We also find that the fibroblasts cultured in collagen-coated dishes are rounder in shape and shorter in perimeter, compared with those cultured in uncoated polystyrene or glass culture dishes. We hypothesise that the rounder cell-shape which weakens the cell-cell nematic contact interaction is responsible for the change in coherence length. A simple mathematical model of the migrating fibroblasts is constructed, which demonstrates that constant motility with weaker nematic interaction strength does indeed lead to the shortening of the coherence length.en
dc.description.notesK.Y. acknowledges support from the Leading Graduate School Promotion Center of Ochanomizu University for a long-term stay at Virginia Tech; she thanks Professor John J. Tyson and his Computational Cell Biology Lab at Virginia Tech for their warm hospitality and the valuable advice she received while this work was conducted. M.G. acknowledges the financial support from JSPS KAKENHI Grant No. 26860144. H.K. acknowledges the financial support from MEXT KAKENHI Grant No. 15H05876 and JSPS KAKENHI Grant No. 18K11464.en
dc.description.sponsorshipLeading Graduate School Promotion Center of Ochanomizu University; JSPS KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [18K11464, 26860144]; MEXT KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [15H05876]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1007/s10867-020-09556-3en
dc.identifier.eissn1573-0689en
dc.identifier.issn0092-0606en
dc.identifier.pmid32860547en
dc.identifier.urihttp://hdl.handle.net/10919/101113en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPattern formationen
dc.subjectCell populationen
dc.subjectFibroblasten
dc.subjectCollagenen
dc.subjectMathematical modelen
dc.titleThe effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formationen
dc.title.serialJournal of Biological Physicsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen
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