eDNA metabarcoding as a means to assess distribution of subterranean fish communities: Iranian blind cave fishes as a case study

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dc.contributor.authorHashemzadeh Segherloo, I.en
dc.contributor.authorTabatabaei, S. N.en
dc.contributor.authorAbdolahi-Mousavi, E.en
dc.contributor.authorHernandez, C.en
dc.contributor.authorNormandaeu, E.en
dc.contributor.authorLaPorte, M.en
dc.contributor.authorAmiri, M.en
dc.contributor.authorRahmati, N. G.en
dc.contributor.authorHallerman, Eric M.en
dc.contributor.authorBernatchez, L.en
dc.coverage.countryIranen
dc.date.accessioned2022-02-06T17:28:56Zen
dc.date.available2022-02-06T17:28:56Zen
dc.date.issued2021-09-22en
dc.date.updated2022-02-06T17:28:53Zen
dc.description.abstractOne of the most important steps in conservation of the subterranean life forms is to decipher their distribution and ecology, which is challenging using traditional approaches. Development of an environmental DNA (eDNA) assay provides an efficient means for discovering and monitoring subterranean life forms. In this study, the distribution of three Iranian blind cave fish species (blind Iran cave barb Garra typhlops, blind Lorestan cave barb Garra lorestanensis, and blind cave loach Eidinemacheilus smithi) was assessed using 12S rRNA eDNA metabarcoding performed using MiFish-U PCR primers and preliminary species distribution modeling (SDM) using bioclimatic data. The majority of sampling localities with positive detection of cave barb eDNA fall within suitable habitats in the Zagros Mountains of Iran. Our results revealed that Lorestan and Iran cave barbs have differential distribution patterns, with some extent of habitat overlap in the vicinity of the originally discovered cave barb locality. According to the observed distribution patterns, the blind Lorestan cave barb and cave loach are mostly distributed in habitats close to the Sezar River (Dez River drainage, Iran), and the blind Iran cave barb is distributed towards the west and probably in a few springs in the Karkheh River drainage. Our data support the previously proposed distribution pattern for the cave barbs, in which the species show partial niche separation and reproductive isolation, with the Lorestan cave barb being a water flow-dependent species and the Iran cave barb being a generalist species preferring variable flow rates. We showed eDNA metabarcoding to be a useful approach for ecological surveys of subterranean fish biodiversity with implications for conservation.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/edn3.264en
dc.identifier.orcidHallerman, Eric [0000-0002-5839-858X]en
dc.identifier.urihttp://hdl.handle.net/10919/108161en
dc.identifier.volume2021en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.titleeDNA metabarcoding as a means to assess distribution of subterranean fish communities: Iranian blind cave fishes as a case studyen
dc.title.serialEnvironmental DNAen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dcterms.dateAccepted2021-08-18en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Natural Resources & Environmenten
pubs.organisational-group/Virginia Tech/Natural Resources & Environment/Fish and Wildlife Conservationen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Natural Resources & Environment/CNRE T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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