Effect of hydrogen peroxide and other protease inhibitors on Cryptosporidium parvum excystation and in vitro development

dc.contributor.authorKniel, Kalmia E.en
dc.contributor.authorSumner, Susan S.en
dc.contributor.authorPierson, Merle D.en
dc.contributor.authorZajac, Anne M.en
dc.contributor.authorHackney, Cameron Rajen
dc.contributor.authorFayer, Ronalden
dc.contributor.authorLindsay, David S.en
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentFood Science and Technologyen
dc.date.accessed2014-06-16en
dc.date.accessioned2014-06-20T14:13:12Zen
dc.date.available2014-06-20T14:13:12Zen
dc.date.issued2004-08en
dc.description.abstractThis study was undertaken to observe the effects of hydrogen peroxide on Cryptosporidium parvum oocysts with respect to protease activity in comparison to known protease inhibitors. In assessing the possible mechanisms of action of hydrogen peroxide, treatment effectiveness was analyzed using 3 assays and the potential roles of proteases and cations were considered. Treatment of C. parvum oocysts with hydrogen peroxide inhibited protease activity up to 50% compared with untreated controls. Treatment of oocysts with chemicals that affect sulfhydryls, including N-ethylmaleimide and dithiolthreitol, inhibited protease activity by > 90%. Treatment of oocysts with these chemicals, along with the protease inhibitors, phenylmethylsulfonyl fluoride (PMSF), ethylenediamine-tetraacetic acid, and cystatin, inhibited protease activity as well as in vitro excystation and infection in a cell culture assay. Several mechanisms may result in the successful inhibition of infection and excystation by hydrogen peroxide treatment, including: oxidation of oocyst wall proteins or lipids, chelating of cations necessary for infection, or hydroxyl radical-induced DNA damage to sporozoites, or both.en
dc.description.sponsorshipCSREES USDA Special Food Safety Grant 98-34382-6916en
dc.description.sponsorshipVPI&SU HATCH project 135563en
dc.description.sponsorshipNew Initiative Grant from the Virginia-Maryland Regional College of Veterinary Medicineen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationK. E. Kniel, S. S. Sumner, M. D. Pierson, A. M. Zajac, C. R. Hackney, R. Fayer, and D. S. Lindsay (2004). 'Effect of Hydrogen Peroxide and Other Protease Inhibitors on Cryptosporidium parvum Excystation and In Vitro Development," Journal of Parasitology, Vol. 90, No. 4, pp. 885-888. doi: http://dx.doi.org/10.1645/GE-203R1en
dc.identifier.doihttps://doi.org/10.1645/ge-203r1en
dc.identifier.issn0022-3395en
dc.identifier.urihttp://hdl.handle.net/10919/49038en
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-203R1en
dc.language.isoenen
dc.publisherAmerican Society of Parasitologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectproteolytic activityen
dc.subjectproteinase activityen
dc.subjectserine-proteaseen
dc.subjectoocystsen
dc.subjectsporozoitesen
dc.subjectcellsen
dc.subjectinvasionen
dc.subjectidentificationen
dc.subjectinactivationen
dc.subjectmerozoitesen
dc.subjectparasitologyen
dc.titleEffect of hydrogen peroxide and other protease inhibitors on Cryptosporidium parvum excystation and in vitro developmenten
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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