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Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

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dc.contributor.authorBoutagy, Nabil E.en
dc.contributor.authorRogers, George W.en
dc.contributor.authorPyne, Emily S.en
dc.contributor.authorAli, Mostafa M.en
dc.contributor.authorHulver, Matthew W.en
dc.contributor.authorFrisard, Madlyn I.en
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2016-11-17T20:54:31Zen
dc.date.available2016-11-17T20:54:31Zen
dc.date.issued2015-10-01en
dc.description.abstractSkeletal muscle mitochondria play a specific role in many disease pathologies. As such, the measurement of oxygen consumption as an indicator of mitochondrial function in this tissue has become more prevalent. Although many technologies and assays exist that measure mitochondrial respiratory pathways in a variety of cells, tissue and species, there is currently a void in the literature in regards to the compilation of these assays using isolated mitochondria from mouse skeletal muscle for use in microplate based technologies. Importantly, the use of microplate based respirometric assays is growing among mitochondrial biologists as it allows for high throughput measurements using minimal quantities of isolated mitochondria. Therefore, a collection of microplate based respirometric assays were developed that are able to assess mechanistic changes/adaptations in oxygen consumption in a commonly used animal model. The methods presented herein provide step-bystep instructions to perform these assays with an optimal amount of mitochondrial protein and reagents, and high precision as evidenced by the minimal variance across the dynamic range of each assay.en
dc.description.versionPublished versionen
dc.format.extent? - ? (11) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3791/53216en
dc.identifier.issn1940-087Xen
dc.identifier.issue104en
dc.identifier.urihttp://hdl.handle.net/10919/73471en
dc.language.isoenen
dc.publisherJournal of Visualized Experimentsen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000368572800044&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectDevelopmental Biologyen
dc.subjectIssue 104en
dc.subjectHigh throughputen
dc.subjectrespirometric assaysen
dc.subjectskeletal muscleen
dc.subjectisolated mitochondriaen
dc.subjectmouse modelen
dc.subjectmetabolismen
dc.subjectOXYGEN-CONSUMPTIONen
dc.subjectCELLSen
dc.titleUsing Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurementsen
dc.title.serialJove-Journal of Visualized Experimentsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exerciseen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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