Experimental and theoretical model for the origin of coiling of cellular protrusions around fibers

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dc.contributor.authorSadhu, Raj Kumaren
dc.contributor.authorHernandez-Padilla, Christianen
dc.contributor.authorEisenbach, Samo Peničen
dc.contributor.authorZhang, Lixiaen
dc.contributor.authorVishwasrao, Harshad D.en
dc.contributor.authorBehkam, Baharehen
dc.contributor.authorKonstantopoulos, Konstantinosen
dc.contributor.authorShroff, Harien
dc.contributor.authorIglič, Alešen
dc.contributor.authorPeles, Elioren
dc.contributor.authorNain, Amrinder S.en
dc.contributor.authorGov, Nir S.en
dc.date.accessioned2023-10-17T16:41:48Zen
dc.date.available2023-10-17T16:41:48Zen
dc.date.issued2023-09-12en
dc.description.abstractProtrusions at the leading-edge of a cell play an important role in sensing the extracellular cues during cellular spreading and motility. Recent studies provided indications that these protrusions wrap (coil) around the extracellular fibers. However, the physics of this coiling process, and the mechanisms that drive it, are not well understood. We present a combined theoretical and experimental study of the coiling of cellular protrusions on fibers of different geometry. Our theoretical model describes membrane protrusions that are produced by curved membrane proteins that recruit the protrusive forces of actin polymerization, and identifies the role of bending and adhesion energies in orienting the leading-edges of the protrusions along the azimuthal (coiling) direction. Our model predicts that the cell’s leading-edge coils on fibers with circular cross-section (above some critical radius), but the coiling ceases for flattened fibers of highly elliptical cross-section. These predictions are verified by 3D visualization and quantitation of coiling on suspended fibers using Dual- View light-sheet microscopy (diSPIM). Overall, we provide a theoretical framework, supported by experiments, which explains the physical origin of the coiling phenomenon.en
dc.description.sponsorshipA.S.N. acknowledges partial funding support from the National Science Foundation (NSF, Grant Nos. 1762634, 2119949, and 2107332). The opinions, findings, and conclusions, or recommendations expressed are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. A.S.N. and B.B. acknowledge the Institute of Critical Technologies and Science (ICTAS) and Macromolecules Innovative Institute (MII) at Virginia Tech for their support in conducting this study. H.S. acknowledges the support from the intramural research program of the National Institute of Biomedical Imaging and Bioengineering within the National Institutes of Health. A.I. and S.P. acknowledge the support from the Slovenian Research Agency (ARIS) through Programme No. P2-0232 and projects Nos. J3-3066 and J2-4447. N.S.G. is the incumbent of the Lee andWilliam Abramowitz Professorial Chair of Biophysics, and acknowledges support by the Ben May Center for Theory and Computation, and the Israel Science Foundation (Grant No. 207/ 22). N.S.G. acknowledges the support of the Ilse Katz Institute for Material Sciences and Magnetic Resonance Research. This research is made possible in part by the historic generosity of the Harold Perlman Family.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41467-023-41273-yen
dc.identifier.urihttp://hdl.handle.net/10919/116491en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherNature Researchen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleExperimental and theoretical model for the origin of coiling of cellular protrusions around fibersen
dc.title.serialNature Communicationsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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