Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
| dc.contributor.author | Kasap, Murat | en |
| dc.contributor.author | Fashae, Kayode | en |
| dc.contributor.author | Torol, Sinem | en |
| dc.contributor.author | Kolayli, Fetiye | en |
| dc.contributor.author | Budak, Fatma | en |
| dc.contributor.author | Vahaboglu, Haluk | en |
| dc.contributor.department | Biochemistry | en |
| dc.date.accessioned | 2012-08-24T11:28:16Z | en |
| dc.date.available | 2012-08-24T11:28:16Z | en |
| dc.date.issued | 2010-01-12 | en |
| dc.date.updated | 2012-08-24T11:28:16Z | en |
| dc.description.abstract | Background We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer. Methods Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments. Results By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26. Conclusion This is the first study demonstrated the occurrence of SHV-12 in Nigeria. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Annals of Clinical Microbiology and Antimicrobials. 2010 Jan 12;9(1):1 | en |
| dc.identifier.doi | https://doi.org/10.1186/1476-0711-9-1 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/18850 | en |
| dc.language.iso | en | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.holder | Murat Kasap et al.; licensee BioMed Central Ltd. | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.title | Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria | en |
| dc.title.serial | Annals of Clinical Microbiology and Antimicrobials | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |