Electrospun Scaffolds Functionalized with a Hydrogen Sulfide Donor Stimulate Angiogenesis

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dc.contributor.authorYao, Tianyuen
dc.contributor.authorvan Nunen, Teunen
dc.contributor.authorRivero, Rebecaen
dc.contributor.authorPowell, Chadwicken
dc.contributor.authorCarrazzone, Ryanen
dc.contributor.authorKessels, Lilianen
dc.contributor.authorWieringa, Paul Andrewen
dc.contributor.authorHafeez, Shahzaden
dc.contributor.authorWolfs, Tim G. A. M.en
dc.contributor.authorMoroni, Lorenzoen
dc.contributor.authorMatson, John B.en
dc.contributor.authorBaker, Matthew B.en
dc.date.accessioned2022-11-21T15:11:17Zen
dc.date.available2022-11-21T15:11:17Zen
dc.date.issued2022-06-17en
dc.description.abstractTissue-engineered constructs are currently limited by the lack of vascularization necessary for the survival and integration of implanted tissues. Hydrogen sulfide (H2S), an endogenous signaling gas (gasotransmitter), has been recently reported as a promising alternative to growth factors to mediate and promote angiogenesis in low concentrations. Yet, sustained delivery of H2S remains a challenge. Herein, we have developed angiogenic scaffolds by covalent attachment of an H2S donor to a polycaprolactone (PCL) electrospun scaffold. These scaffolds were engineered to include azide functional groups (on 1, 5, or 10% of the PCL end groups) and were modified using a straightforward click reaction with an alkyne-functionalized N-thiocarboxyanhydride (alkynyl-NTA). This created H2S-releasing scaffolds that rely on NTA ring-opening in water followed by conversion of released carbonyl sulfide into H2S. These functionalized scaffolds showed dose-dependent release of H2S based on the amount of NTA functionality within the scaffold. The NTA-functionalized fibrous scaffolds supported human umbilical vein endothelial cell (HUVEC) proliferation, formed more confluent endothelial monolayers, and facilitated the formation of tight cell-cell junctions to a greater extent than unfunctionalized scaffolds. Covalent conjugation of H2S donors to scaffolds not only promotes HUVEC proliferation in vitro, but also increases neovascularization in ovo, as observed in the chick chorioallantoic membrane assay. NTA-functionalized scaffolds provide localized control over vascularization through the sustained delivery of a powerful endogenous angiogenic agent, which should be further explored to promote angiogenesis in tissue engineering.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1021/acsami.2c06686en
dc.identifier.eissn1944-8252en
dc.identifier.issn1944-8244en
dc.identifier.issue25en
dc.identifier.pmid35715217en
dc.identifier.urihttp://hdl.handle.net/10919/112685en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectelectrospunen
dc.subjectN-thiocarboxyanhydridesen
dc.subjectclick functionalizationen
dc.subjectangiogenesisen
dc.subjectreactive sulfur speciesen
dc.titleElectrospun Scaffolds Functionalized with a Hydrogen Sulfide Donor Stimulate Angiogenesisen
dc.title.serialACS Applied Materials & Interfacesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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