Identification of soil bacteria capable of utilizing a corn ethanol fermentation byproduct

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dc.contributor.authorPackard, Hollyen
dc.contributor.authorTaylor, Zachary W.en
dc.contributor.authorWilliams, Stephanie L.en
dc.contributor.authorGuimarães, Pedro Ivoen
dc.contributor.authorToth, Jacksonen
dc.contributor.authorJensen, Roderick V.en
dc.contributor.authorSenger, Ryan S.en
dc.contributor.authorKuhn, David D.en
dc.contributor.authorStevens, Ann M.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentBiological Systems Engineeringen
dc.contributor.departmentChemical Engineeringen
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2019-04-01T17:18:03Zen
dc.date.available2019-04-01T17:18:03Zen
dc.date.issued2019-03-08en
dc.description.abstractA commercial corn ethanol production byproduct (syrup) was used as a bacterial growth medium with the long-term aim to repurpose the resulting microbial biomass as a protein supplement in aquaculture feeds. Anaerobic batch reactors were used to enrich for soil bacteria metabolizing the syrup as the sole nutrient source over an eight-day period with the goal of obtaining pure cultures of facultative organisms from the reactors. Amplification of the V4 variable region of the 16S rRNA gene was performed using barcoded primers to track the succession of microbes enriched for during growth on the syrup. The resulting PCR products were sequenced using Illumina MiSeq protocols, analyzed via the program QIIME, and the alpha-diversity was calculated. Seven bacterial families were the most prevalent in the bioreactor community after eight days of enrichment: Clostridiaceae, Alicyclobacillaceae, Ruminococcaceae, Burkholderiaceae, Bacillaceae, Veillonellaceae, and Enterobacteriaceae. Pure culture isolates obtained from the reactors, and additional laboratory stock strains, capable of facultative growth, were grown aerobically in microtiter plates with the syrup substrate to monitor growth yield. Reactor isolates of interest were identified at a species level using the full 16S rRNA gene and other biomarkers. Bacillus species, commonly used as probiotics in aquaculture, showed the highest biomass yield of the monocultures examined. Binary combinations of monocultures yielded no apparent synergism between organisms, suggesting competition for nutrients instead of cooperative metabolite conversion. © 2019 Packard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0212685en
dc.identifier.issn19326203en
dc.identifier.issue3en
dc.identifier.othere0212685en
dc.identifier.pmid30849084en
dc.identifier.urihttp://hdl.handle.net/10919/88794en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherPLoSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIdentification of soil bacteria capable of utilizing a corn ethanol fermentation byproducten
dc.title.serialPLoS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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