Evidence for continuous potential for gene transcription during the cell cycle of a eukaryote

dc.contributor.authorBaechtel, F. Samuelen
dc.contributor.departmentBiochemistry and Nutritionen
dc.date.accessioned2019-10-10T19:27:50Zen
dc.date.available2019-10-10T19:27:50Zen
dc.date.issued1970en
dc.description.abstractSynchronous cultures of Chlorella pyrenoidosa (strain 7- 11-05) have been utilized to measure the potential expression of the structural gene for isocitrate lyase (three D<sub>S</sub>-Isocitrate glyoxylate-lyase, EC 4.1.3.1) during the cell cycle. Synthesis of the enzyme could be induced by placing cultures in the dark on acetate, with the induction process occurring in a quadratic fashion. By addition of cycloheximide during the course of induction, the increase in isocitrate lyase activity was shown to result from de novo protein synthesis. In the absence of protein synthesis the enzyme was stable for at least five hours. The pattern of uninduced isocitrate lyase synthesis during the cell cycle in continuous light, paralleled the stepwise increase of total cellular DNA. The enzyme appeared to be fully repressed for most of the cell cycle, and was derepressed during the time of DNA replication. Isocitrate lyase could be induced at all times in the cell cycle, indicating that the potential for gene expression is continuous in this eukaryote. A time lag was observed between the beginning of DNA replication and the initial rise in potential for isocitrate lyase gene expression. The control of gene expression in Chlorella appeared to be similar to that found in a fission yeast.en
dc.description.degreePh. D.en
dc.format.extentiv, 44 leavesen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/94523en
dc.language.isoen_USen
dc.publisherVirginia Polytechnic Instituteen
dc.relation.isformatofOCLC# 22440836en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1970.B3en
dc.subject.lcshEukaryotic cellsen
dc.titleEvidence for continuous potential for gene transcription during the cell cycle of a eukaryoteen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Nutritionen
thesis.degree.grantorVirginia Polytechnic Instituteen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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