Mini-review: In vitro Metabolic Engineering for Biomanufacturing of High-value Products

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dc.contributorVirginia Techen
dc.contributor.authorGuo, Weihuaen
dc.contributor.authorSheng, Jiayuanen
dc.contributor.authorFeng, Xueyangen
dc.contributor.departmentBiological Systems Engineeringen
dc.date.accessioned2017-03-28T17:31:56Zen
dc.date.available2017-03-28T17:31:56Zen
dc.date.issued2017-01-19en
dc.description.abstractWith the breakthroughs in biomolecular engineering and synthetic biology, many valuable biologically active compound and commodity chemicals have been successfully manufactured using cell-based approaches in the past decade. However, because of the high complexity of cell metabolism, the identification and optimization of rate-limiting metabolic pathways for improving the product yield is often difficult, which represents a significant and unavoidable barrier of traditional in vivo metabolic engineering. Recently, some in vitro engineering approaches were proposed as alternative strategies to solve this problem. In brief, by reconstituting a biosynthetic pathway in a cell-free environment with the supplement of cofactors and substrates, the performance of each biosynthetic pathway could be evaluated and optimized systematically. Several value-added products, including chemicals, nutraceuticals, and drug precursors, have been biosynthesized as proof-of-concept demonstrations of in vitro metabolic engineering. This mini-review summarizes the recent progresses on the emerging topic of in vitro metabolic engineering and comments on the potential application of cell-free technology to speed up the “design-build-test” cycles of biomanufacturing.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.csbj.2017.01.006en
dc.identifier.urihttp://hdl.handle.net/10919/76698en
dc.identifier.volume15en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCell-freeen
dc.subjectBiosynthesisen
dc.subjectMetabolic pathwaysen
dc.subjectDesign-build-test cycleen
dc.titleMini-review: In vitro Metabolic Engineering for Biomanufacturing of High-value Productsen
dc.title.serialComputational and Structural Biotechnology Journalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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