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Molecular Cloning and Characterization of the First Caspase in the Striped Stem Borer, Chilo suppressalis

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dc.contributor.authorLu, Ming-Xingen
dc.contributor.authorDu, Yu-Zhouen
dc.contributor.authorCao, Shuang-Shuangen
dc.contributor.authorLiu, Pingyangen
dc.contributor.authorLi, Jianyongen
dc.contributor.departmentBiochemistryen
dc.date.accessed2014-01-06en
dc.date.accessioned2014-01-15T14:16:35Zen
dc.date.available2014-01-15T14:16:35Zen
dc.date.issued2013-05-15en
dc.description.abstractApoptosis is executed through the activity of the caspases that are aspartyl-specific proteases. In this study, we isolated the caspase gene (Cscaspase-1) of Chilo suppressalis (one of the leading pests responsible for destruction of rice crops). It possesses the open reading frame (ORF) of 295 amino acids including prodomain, large subunit and small subunits, and two cleavage sites (Asp23 and Asp194) were found to be located among them. In addition to these profiles, Cscaspase-1 contains two active sites (His134 and Cys176). Genomic analysis demonstrated there was no intron in the genome of Cscaspase-1. The Cscaspase-1 transcripts were found in all tissues of the fifth instar larvae, and higher levels were found in the midgut, hindgut and Malpighian tubules. Examination of Cscaspase-1 expression in different developmental stages indicated low constitutive levels in the eggs and early larvae stages, and higher abundances were exhibited in the last larvae and pupae stages. The relative mRNA levels of Cscaspase-1 were induced by heat and cold temperatures. For example, the highest increase of Cscaspase-1 transcription was at -3 C and 36 C respectively. In a word, Cscaspase-1 plays a role of effector in the apoptosis of C. suppressalis. It also correlates with development, metamorphosis and thermotolerance of C. suppreassalis.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLu, Ming-Xing; Du, Yu-Zhou; Cao, Shuang-Shuang; Liu, Pingyang; Li, Jianyong. 2013. "Molecular Cloning and Characterization of the First Caspase in the Striped Stem Borer, Chilo suppressalis." Int. J. Mol. Sci. 2013, 14(5), 10229-10241; doi:10.3390/ijms140510229en
dc.identifier.doihttps://doi.org/10.3390/ijms140510229en
dc.identifier.issn1422-0067en
dc.identifier.urihttp://hdl.handle.net/10919/24845en
dc.identifier.urlhttp://www.mdpi.com/1422-0067/14/5/10229en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectApoptosisen
dc.subjectCscaspase-1en
dc.subjectChilo suppressalisen
dc.subjectexpressionen
dc.subjectdevelopmenten
dc.subjectthermotoleranceen
dc.titleMolecular Cloning and Characterization of the First Caspase in the Striped Stem Borer, Chilo suppressalisen
dc.title.serialInternational Journal of Molecular Sciencesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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