Isolation, molecular characterization, and in vitro schizogonic development of Sarcocystis sp ex Accipiter cooperii from a naturally infected Cooper's hawk (Accipiter cooperii)

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dc.contributor.authorLindsay, David S.en
dc.contributor.authorVerma, Shiv K.en
dc.contributor.authorScott, Daviden
dc.contributor.authorDubey, Jitender P.en
dc.contributor.authorvon Dohlen, Alexa Rosypalen
dc.date.accessioned2020-03-24T19:31:55Zen
dc.date.available2020-03-24T19:31:55Zen
dc.date.issued2017-04en
dc.description.abstractRaptors serve as the definitive host for several Sarcocystis species. The complete life cycles of only a few of these Sarcocystis species that use birds of prey as definitive hosts have been described. In the present study, Sarcocystis species sporocysts were obtained from the intestine of a Cooper's hawk (Accipiter cooperii) and were used to infect cell cultures of African green monkey kidney cells to isolate a continuous culture and describe asexual stages of the parasite. Two clones of the parasite were obtained by limiting dilution. Asexual stages were used to obtain DNA for molecular classification and identification. PCR amplification and sequencing were done at three nuclear ribosomal DNA loci; 18S rRNA, 28S rRNA, and ITS-1, and the mitochondrial cytochrome c oxidase subunit 1 (cox1) locus. Examination of clonal isolates of the parasite indicated a single species related to S. columbae (termed Sarcocystis sp. ex Accipiter cooperii) was present in the Cooper's hawk. Our results document for the first time Sarcocystis sp. ex A. cooperii occurs naturally in an unknown intermediate host in North America and that Cooper's hawks (A. cooperii) are a natural definitive host. (C) 2016 Elsevier Ireland Ltd. All rights reserved.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis work was supported by grant # 1505407 from the National Science Foundation Historically Black Colleges and Universities Undergraduate Program to ACR and an IRC grant from the Virginia Maryland College of Veterinary Medicine to DSL.en
dc.description.sponsorshipNational Science Foundation Historically Black Colleges and Universities Undergraduate Program [1505407]; Virginia Maryland College of Veterinary Medicineen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.parint.2016.12.002en
dc.identifier.issn1383-5769en
dc.identifier.issue2en
dc.identifier.pmid28033565en
dc.identifier.urihttp://hdl.handle.net/10919/97454en
dc.identifier.volume66en
dc.language.isoenen
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectCooper's hawken
dc.subjectAccipiter cooperiien
dc.subjectCell cultureen
dc.subjectSarcocystis sp ex A. cooperiien
dc.subjectSarcocystis columbaeen
dc.subjectSporocystsen
dc.subjectRaptoren
dc.titleIsolation, molecular characterization, and in vitro schizogonic development of Sarcocystis sp ex Accipiter cooperii from a naturally infected Cooper's hawk (Accipiter cooperii)en
dc.title.serialParasitology Internationalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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