Profiling intestinal stem and proliferative cells in the small intestine of broiler chickens via in situ hybridization during the peri-hatch period

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dc.contributor.authorCloft, Sara E.en
dc.contributor.authorUni, Zehavaen
dc.contributor.authorWong, Eric A.en
dc.date.accessioned2023-10-19T13:19:16Zen
dc.date.available2023-10-19T13:19:16Zen
dc.date.issued2023-04en
dc.description.abstractMature small intestines have crypts populated by stem cells which produce replacement cells to maintain the absorptive villus surface area. The embryonic crypt is rudimentary and cells along the villi are capable of proliferation. By 7 d post-hatch the crypts are developed and are the primary sites of proliferation. Research characterizing the proliferative expansion of the small intestine during the peri-hatch period is lacking. The objective of this study was to profile the changes of genes that are markers of stem cells and proliferation: Olfactomedin 4 (Olfm4), Leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5), and marker of proliferation Ki67 from embryonic day 17 to 7 d post-hatch using quantitative PCR and in situ hybridization (ISH). The expression of the stem cell marker genes differed. Olfm4 mRNA increased while Lgr5 mRNA decreased post-hatch. Ki67 mRNA decreased post-hatch in the duodenum and was generally the greatest in the ileum. The ISH was consistent with the quantitative PCR results. Olfm4 mRNA was only seen in the crypts and increased with morphological development of the crypts. In contrast Lgr5 mRNA was expressed in the crypt and the villi in the embryonic periods but became restricted to the intestinal crypt during the post-hatch period. Ki67 mRNA was expressed throughout the intestine pre-hatch, but then expression became restricted to the crypt and the center of the villi. The ontogeny of Olfm4, Lgr5, and Ki67 expressing cells show that proliferation in the peri-hatch intestine changes from along the entire villi to being restricted within the crypts.en
dc.description.notesThis work was supported in part by Grant No. US- 5074-18CR from the United States - Israel Binational Agricultural Research and Development (BARD) Fund, the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture (NIFA- USDA) and the Virginia Tech Open Access Subvention Fund.en
dc.description.sponsorshipUnited States - Israel Binational Agricultural Research and Development (BARD) Fund [US- 5074-18CR]; Virginia Agricultural Experiment Station; Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture (NIFA- USDA); Virginia Tech Open Access Subvention Funden
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.psj.2023.102495en
dc.identifier.eissn1525-3171en
dc.identifier.issn0032-5791en
dc.identifier.issue4en
dc.identifier.other102495en
dc.identifier.pmid36758370en
dc.identifier.urihttp://hdl.handle.net/10919/116511en
dc.identifier.volume102en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsmall intestineen
dc.subjectstem cellen
dc.subjectLgr5en
dc.subjectOlfm4en
dc.subjectKi67en
dc.titleProfiling intestinal stem and proliferative cells in the small intestine of broiler chickens via in situ hybridization during the peri-hatch perioden
dc.title.serialPoultry Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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