Maize dwarf mosaic virus: purification, some physical properties, and yield of virus from several corn hybrids

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dc.contributor.authorJones, Ronald K.en
dc.contributor.committeechairTolin, Sue A.en
dc.contributor.committeememberHale, Maynard G.en
dc.contributor.committeememberGenter, C. F.en
dc.contributor.committeememberRoane, Curtis W.en
dc.contributor.committeememberFox, J. A.en
dc.contributor.committeememberStipes, R. Jayen
dc.contributor.departmentPlant Pathologyen
dc.date.accessioned2014-03-14T21:10:18Zen
dc.date.adate2010-04-07en
dc.date.available2014-03-14T21:10:18Zen
dc.date.issued1971-04-05en
dc.date.rdate2010-04-07en
dc.date.sdate2010-04-07en
dc.description.abstractTo better understand the nature of maize dwarf mosaic virus (MDMV) and its interaction with susceptible and resistant corn hybrids, the purification of MDMV and its physical properties were re-evaluated and a rapid assay procedure was developed. The virus was purified by chloroform clarification and differential and sucrose density-gradient centrifugation. Corn tissue was homogenized in 0.1 M sodium citrate plus 0.5% mercaptoethanol. The virus was resuspended after high speed centrifugation in 0.005 M sodium citrate, pH 7.0. Purified virus has an ultraviolet absorption spectrum typical for filamentous viruses having 5% RNA, shows birefringence, has an A260/280 of 1.18, and a sedimentation coefficient of 160-162 S. The virus is filamentous and appears to be a member of the potato virus Y group. A yield of 9.0-23.0 llg of virus/g fresh weigh.t of field-grown, mechanically inoculated Hy X Cl03 was determined by analytical sucrose density-gradient centrifugation. This represents a yield of purified virus of approximately 25% of that in the clarified sap. Results obtained from the density-gradient analysis were comparable to but more sensitive than those from the microprecipitin test and a systemic infectivity assay. The systemic infectivity assay on corn, analyzed by maximum likelihood and log log transformation, showed a yield of purified virus of 0.4% of that in the crude sap.en
dc.description.degreePh. D.en
dc.format.extentvi, 53 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-04072010-020146en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04072010-020146/en
dc.identifier.urihttp://hdl.handle.net/10919/37559en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1971.J66.pdfen
dc.relation.isformatofOCLC# 07938141en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectvirusesen
dc.subject.lccLD5655.V856 1971.J66en
dc.titleMaize dwarf mosaic virus: purification, some physical properties, and yield of virus from several corn hybridsen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplinePlant Pathologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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