Maternal Germline-Specific Genes in the Asian Malaria Mosquito Anopheles stephensi: Characterization and Application for Disease Control

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dc.contributor.authorBiedler, James K.en
dc.contributor.authorQi, Yi,omen
dc.contributor.authorPledger, DAviden
dc.contributor.authorJames, Anthony A.en
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.departmentBiochemistryen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2018-01-15T05:29:55Zen
dc.date.available2018-01-15T05:29:55Zen
dc.date.issued2015-02-01en
dc.description.abstractAnopheles stephensi is a principal vector of urban malaria on the Indian subcontinent and an emerging model for molecular and genetic studies of mosquito biology. To enhance our understanding of female mosquito reproduction, and to develop new tools for basic research and for genetic strategies to control mosquitoborne infectious diseases, we identified 79 genes that displayed previtellogenic germline-specific expression based on RNA-Seq data generated from 11 life stage–specific and sex-specific samples. Analysis of this gene set provided insights into the biology and evolution of female reproduction. Promoters from two of these candidates, vitellogenin receptor and nanos, were used in independent transgenic cassettes for the expression of artificial microRNAs against suspected mosquito maternal-effect genes, discontinuous actin hexagon and myd88. We show these promoters have early germline-specific expression and demonstrate 73% and 42% knockdown of myd88 and discontinuous actin hexagon mRNA in ovaries 48 hr after blood meal, respectively. Additionally, we demonstrate maternal-specific delivery of mRNA and protein to progeny embryos. We discuss the application of this system of maternal delivery of mRNA/miRNA/protein in research on mosquito reproduction and embryonic development, and for the development of a gene drive system based on maternal-effect dominant embryonic arrest.en
dc.description.versionPublished versionen
dc.format.extent157 - 166 (10) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1534/g3.114.015578en
dc.identifier.issn2160-1836en
dc.identifier.issue2en
dc.identifier.urihttp://hdl.handle.net/10919/81777en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherGenetics Society of Americaen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000349451500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 3.0 Unporteden
dc.rights.holderThe Author(s)en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectGenetics & Heredityen
dc.subjectvector controlen
dc.subjectvector-borne diseaseen
dc.subjecttranscriptomeen
dc.subjectAn. stephensien
dc.subjectgene driveen
dc.subjectMAJOR ARBOVIRUS VECTORen
dc.subjectDNA COPY NUMBERen
dc.subjectAEDES-AEGYPTIen
dc.subjectEXPRESSION ANALYSISen
dc.subjectREGULATORY REGIONen
dc.subjectGENOME SEQUENCEen
dc.subjectRNA-SEQen
dc.subjectDROSOPHILAen
dc.subjectGAMBIAEen
dc.subjectTRANSFORMATIONen
dc.titleMaternal Germline-Specific Genes in the Asian Malaria Mosquito Anopheles stephensi: Characterization and Application for Disease Controlen
dc.title.serialG3-Genes Genomes Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Facultyen

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