Quantitative In Vitro Characterization of Membrane Permeability for Electroporated Mammalian Cells

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dc.contributor.authorSweeney, Daniel C.en
dc.contributor.committeechairDavalos, Rafael V.en
dc.contributor.committeechairCimini, Danielaen
dc.contributor.committeememberVerbridge, Scotten
dc.contributor.committeememberPoelzing, Stevenen
dc.contributor.committeememberSafaai-Jazi, Ahmaden
dc.contributor.departmentDepartment of Biomedical Engineering and Mechanicsen
dc.date.accessioned2019-10-09T06:00:20Zen
dc.date.available2019-10-09T06:00:20Zen
dc.date.issued2018-04-16en
dc.description.abstractElectroporation-based treatments are motivated by the response of biological membranes to high- intensity pulsed electric fields. These fields rearrange the membrane structure to enhance the membrane's diffusive permeability, or the degree to which a membrane allows molecules to diffuse through it, is impacted by the structure, composition, and environment in which the cell resides. Tracer molecules have been developed that are unable to pass through intact cell membranes yet enter permeabilized cells. This dissertation investigates the hypothesis that the flow of such molecules may be used to quantify the effects of the electrical stimulus and environmental conditions leading to membrane electroporation. Specifically, a series of electrical pulses that alternates between positive and negative pulses permeabilizes cells more symmetrically than a longer pulse with the same total on-time. However, the magnitude of this symmetric entry decreases for the shorter alternating pulses. Furthermore, a method for quantitatively measuring the permeability of the cell membrane was proposed and validated. From data near the electroporation threshold, the response of cells varies widely in the manner in which cells become permeabilized. This method is applied to study the transient cell membrane permeability induced by electroporation and is used to demonstrate that the cell membrane remains permeable beyond 30 min following treatment. To analyze these experimental findings in the context of physical mechanisms, computational models of molecular uptake were developed to simulate electroporation. The results of these simulations indicate that the cell's local environment during electroporation facilitates the degree of molecular uptake. We use these models to predict how manipulating both the environment of cells during electroporation affects the induced membrane permeability. These experimental and computational results provide evidence that supports the hypothesis of this dissertation and provide a foundation for future investigation and simulation of membrane electroporation.en
dc.description.degreePHDen
dc.format.mediumETDen
dc.identifier.othervt_gsexam:14506en
dc.identifier.urihttp://hdl.handle.net/10919/94416en
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectbioelectricsen
dc.subjectelectroporationen
dc.subjectbiotransporten
dc.subjectquantitative microscopyen
dc.subjectmembrane permeabilityen
dc.subjectpulsed electric fieldsen
dc.titleQuantitative In Vitro Characterization of Membrane Permeability for Electroporated Mammalian Cellsen
dc.typeDissertationen
thesis.degree.disciplineBiomedical Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePHDen

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