Shiga Toxin Subtypes, Serogroups, Phylogroups, RAPD Genotypic Diversity, and Select Virulence Markers of Shiga-Toxigenic Escherichia coli Strains from Goats in Mid-Atlantic US

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dc.contributor.authorNdegwa, Euniceen
dc.contributor.authorO'Brien, Dahliaen
dc.contributor.authorMatthew, Kwameen
dc.contributor.authorWang, Zhenpingen
dc.contributor.authorKim, Jiminen
dc.date.accessioned2022-11-21T13:07:11Zen
dc.date.available2022-11-21T13:07:11Zen
dc.date.issued2022-09-15en
dc.date.updated2022-11-20T20:31:48Zen
dc.description.abstractUnderstanding Shiga toxin subtypes in <i>E. coli</i> from reservoir hosts may give insight into their significance as human pathogens. The data also serve as an epidemiological tool for source tracking. We characterized Shiga toxin subtypes in 491 goat <i>E. coli</i> isolates (STEC) from the mid-Atlantic US region (<i>stx1</i> = 278, <i>stx2</i> = 213, and <i>stx1</i>/<i>stx2</i> = 95). Their serogroups, phylogroups, M13RAPD genotypes, <i>eae</i> (intimin), and <i>hly</i> (hemolysin) genes were also evaluated. STEC-positive for <i>stx1</i> harbored <i>Stx1c</i> (79%), <i>stx1a</i> (21%), and <i>stx a</i>/<i>c</i> (4%). Those positive for <i>Stx2</i> harbored <i>stx2a</i> (55%) and <i>Stx2b</i> (32%), while <i>stx2a/stx2d</i> and <i>stx2a/stx2b</i> were each 2%. Among the 343 STEC that were serogrouped, 46% (n = 158) belonged to O8, 20% (n = 67) to 076, 12% (n = 42) to O91, 5% (n = 17) to O5, and 5% (n = 18) to O26. Less than 5% belonged to O78, O87, O146, and O103. The <i>hly</i> and <i>eae</i> genes were detected in 48% and 14% of STEC, respectively. Most belonged to phylogroup B1 (73%), followed by D (10%), E (8%), A (4%), B2 (4%), and F (1%). M13RAPD genotyping revealed clonality of 091, O5, O87, O103, and O78 but higher diversity in the O8, O76, and O26 serogroups. These results indicate goat STEC belonged to important non-O157 STEC serogroups, were genomically diverse, and harbored Shiga toxin subtypes associated with severe human disease.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3390/microorganisms10091842en
dc.identifier.eissn2076-2607en
dc.identifier.issn2076-2607en
dc.identifier.issue9en
dc.identifier.otherPMC9505625en
dc.identifier.othermicroorganisms10091842 (PII)en
dc.identifier.pmid36144444en
dc.identifier.urihttp://hdl.handle.net/10919/112682en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/36144444en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSTECen
dc.subjectShiga toxin subtypesen
dc.subjectgoatsen
dc.subjectphylogroupsen
dc.subjectserogroupsen
dc.subjectvirulence genesen
dc.subjectDigestive Diseasesen
dc.titleShiga Toxin Subtypes, Serogroups, Phylogroups, RAPD Genotypic Diversity, and Select Virulence Markers of Shiga-Toxigenic <i>Escherichia coli</i> Strains from Goats in Mid-Atlantic USen
dc.title.serialMicroorganismsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherresearch-articleen
dc.type.otherJournal Articleen
dcterms.dateAccepted2022-09-13en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen

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